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BioMed Research International
Volume 2015 (2015), Article ID 265425, 6 pages
Research Article

Evaluation of Antibacterial Activity of Some Traditionally Used Medicinal Plants against Human Pathogenic Bacteria

1Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu, Nepal
2Nepal Academy of Science and Technology (NAST), Khumaltar, Lalitpur, Nepal
3Department of Pathobiological Science, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA
4Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
5Patan Hospital, Patan, Lalitpur, Nepal

Received 22 October 2014; Accepted 16 January 2015

Academic Editor: Frederick D. Quinn

Copyright © 2015 Bishnu P. Marasini et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The elaborated steps for the isolation and processing of the plant materials are covered in Supplementary Material. The antibiotic resistance pattern of tested bacteria is shown in Supplemental Table 1. The MIC values were determined by microbroth dilution method using 96-well plates (detailed procedure is in Supplementary Material). The MDR and non-MDR clinical strains used in this work were isolated from human infections diagnosed in a tertiary care hospital of Nepal (Supplemental Table 1). However, these extracts showed the larger inhibition zone (by agar-well diffusion method, Supplemental Table 2). The minimum bactericidal concentration (MBC) values of the plant extracts against tested bacterial species were found to be largely higher and ranged from 98 to >25,000 𝜇g/mL (Supplemental Table 3). In our study, the plant extracts from C. longa, G. biloba, and R. serpentina were demonstrated to inhibit the growth of all tested Gram-positive bacteria, whereas only the plant extract from C. dactylon was observed to inhibit the growth of all tested Gram-negative bacteria except C. freundii (Supplemental Table 2).

  1. Supplementary Material