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BioMed Research International
Volume 2015, Article ID 267538, 5 pages
Research Article

Improved Sugar Production by Optimizing Planetary Mill Pretreatment and Enzyme Hydrolysis Process

1Korea Institute of Ceramic Engineering and Technology (KICET), 101 Soho-ro, Jinju-si, Gyeongsangnam-do 52851, Republic of Korea
2Department of Chemical Engineer, Hanyang University, Seoul 04763, Republic of Korea
3School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan 44919, Republic of Korea
4Department of Forest Products and Technology, Chonnam National University, Gwangju 61186, Republic of Korea

Received 19 May 2015; Revised 30 July 2015; Accepted 9 August 2015

Academic Editor: Yu Zhang

Copyright © 2015 Jeong Heo Kwon et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The effects of three single variable parameters on the glucose yields were investigated before measuring the combinational effects of experimental conditions. Figure S1 shows the effects each of the individual variables had on the glucose yields. As increasing the value of each variable, the productions of glucose were increased and reached to saturation points. Finally, optimal values for single variable were found at 100 minutes, 16FPU/g-biomass, and 12 hours for milling time, enzyme quantity and reaction time, respectively (Fig S1). The standard values for the locked parameters were milling for 8 hours, 42.5 FPU/g-biomass and a enzymatic hydrolysis time of 72 hours.

After planetary milling, the sizes of biomass were reduced. However, there was no significant difference of biomass size depending on milling time. When it compared with non-pretreated sample, it was clear that planetary mill disrupted the rigid surface and its intact phytoliths (Fig S2). Figure S2 shows scanning electron micrographs of before (a) and after 40 (b), 60 (c) and 120min (d) planetary milled pitch pine wood wastes. Small pieces of non-milling sample were selected to obtain SEM image. Even though the size of biomass does not homogenous, planetary mill was effective to reduce biomass size.

  1. Supplementary Material