Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2015, Article ID 270973, 12 pages
http://dx.doi.org/10.1155/2015/270973
Research Article

In Vitro and In Vivo Cytogenotoxic Effects of Hot Aqueous Extract of Achyrocline satureioides (Lam.) DC.

1Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36, Km 601, Río Cuarto, C5800 Córdoba, Argentina
2Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Rivadavia 1917, C1033AAJ Buenos Aires, Argentina
3Área de Microscopía Electrónica, Universidad Nacional de Río Cuarto, Ruta 36, Km 601, Río Cuarto, C5800 Córdoba, Argentina
4Farmacognosia, Departamento de Farmacia, Universidad Nacional de Córdoba (IMBIV-CONICET), Ciudad Universitaria, C5000 Córdoba, Argentina

Received 26 December 2014; Revised 1 February 2015; Accepted 1 February 2015

Academic Editor: Isabel C. F. R. Ferreira

Copyright © 2015 L. N. Cariddi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In this work we extend the toxicological studies of hot aqueous extract of A. satureioides (As-HAE) evaluating cytotoxic and apoptotic effects on human peripheral blood mononuclear cells (PBMCs). We also determine genotoxic action of this extract in vivo. In addition, the extract was chemically characterized. Finally, we established a comparison with previous data of cold aqueous extract. The As-HAE induced cytotoxicity on PBMCs determined by trypan blue dye exclusion (CC50 = 653 μg/mL) and MTT (CC50 = 588 μg/mL) assays being more toxic than cold extract. However, As-HAE as well as cold extract did not induce apoptosis measured by Hoechst 33258 staining, TUNEL assay, and DNA fragmentation analysis. The in vivo micronucleus test showed that As-HAE exerted cytogenotoxic effects on bone marrow of mice, contrary to what was observed with cold extract. The chemical study of As-HAE allowed identifying the flavonoids found in cold extract: luteolin, quercetin, and 3-O-methylquercetin, but at higher concentrations. We suggest that toxic effects induced by As-HAE could be due to high concentrations of these flavonoids. Given that As-HAE is the most used in folkloric medicine, its administration should be controlled in order to prevent potential cell damage.