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Figure 2: Microphotographs of the nuclear morphology of normal human PBMCs treated with hot aqueous extract of Achyrocline satureioides (As-HAE) and stained with Hoechst 33258 (100x). (a) Medium alone (control), (b) cells treated with hydrogen peroxide (1 mmol/L), (c) As-HAE (10 μg/mL), (d) As-HAE (600 μg/mL), and (e) As-HAE (1000 μg/mL). Arrows show apoptotic cells. These cells were identified by characteristic features of apoptosis (e.g., nuclear condensation, membrane blebs formation, and apoptotic bodies).