Candesartan Mediated Amelioration of Cisplatin-Induced Testicular Damage Is Associated with Alterations in Expression Patterns of Nephrin and Podocin
Immunofluorescence double staining for nephrin and podocin in the testes of rats following treatment with cisplatin and/or candesartan. Nephrin was stained in green with FITC, podocin was stained in red with Texas Red, and the nuclei were stained in blue with DAPI. The overlaps of nephrin and podocin are indicated in orange. (a) Representative findings of immunofluorescence double staining and changes in each group. (b) High magnification images of control testis. Arrows indicate that nephrin and podocin are coexpressed in linear formation near basal membrane of seminiferous tubules. (c) High magnification images of testis following treatment with cisplatin. Arrows indicate that, after cisplatin (7 mg) administration, the expression patterns of nephrin and podocin were dissociated and in discrete granular structure. (d) Quantitative analysis of immunofluorescence positive cells for nephrin and podocin. †, , and differ from Group 1, Group 3, and Group 5, respectively ().