BioMed Research International / 2015 / Article / Fig 6

Research Article

Candesartan Mediated Amelioration of Cisplatin-Induced Testicular Damage Is Associated with Alterations in Expression Patterns of Nephrin and Podocin

Figure 6

Changes in expression levels of nephrin and podocin in cultured mouse Sertoli cells, line TM4, following treatment with angiotensin-II and/or candesartan. (a) Total RNA was extracted from TM4 cells and analyzed for nephrin mRNA levels by real-time RT-PCR. Each column represents the mean value with standard deviation. differs from TM4 cells treated with cisplatin alone (). (b) Total RNA was extracted from the testes and analyzed for podocin mRNA levels by real-time RT-PCR. Each column represents the mean value with standard deviation. differs from TM4 cells treated with angiotensin-II alone (). (c) Protein was extracted from TM4 cells and expression levels of nephrin, podocin, and β-actin were analyzed by Western blotting.
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