Cinnabar-Induced Subchronic Renal Injury Is Associated with Increased Apoptosis in Rats

Wang, Ying; Wang, Dapeng; Wu, Jie; Wang, Bohan; Gao, Xianhui; Wang, Liangjun; Ma, Honglin

doi:https://doi.org/10.1155/2015/278931

BioMed Research International

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Research Article | Open Access

Volume 2015 | Article ID 278931 | https://doi.org/10.1155/2015/278931

Cinnabar-Induced Subchronic Renal Injury Is Associated with Increased Apoptosis in Rats

Ying Wang,¹Dapeng Wang,¹Jie Wu,¹Bohan Wang,¹Xianhui Gao,¹Liangjun Wang,¹and Honglin Ma¹

Academic Editor: Rita Rezzani

Received04 Jun 2014

Accepted26 Sept 2014

Published06 Jan 2015

Abstract

The aim of this study was to explore the role of apoptosis in cinnabar-induced renal injury in rats. To test this role, rats were dosed orally with cinnabar (1 g/kg/day) for 8 weeks or 12 weeks, and the control rats were treated with 5% carboxymethylcellulose solution. Levels of urinary mercury (UHg), renal mercury (RHg), serum creatinine (SCr), and urine kidney injury molecule 1 (KIM-1) were assessed, and renal pathology was analyzed. Apoptotic cells were identified and the apoptotic index was calculated. A rat antibody array was used to analyze expression of cytokines associated with apoptosis. Results from these analyses showed that UHg, RHg, and urine KIM-1, but not SCr, levels were significantly increased in cinnabar-treated rats. Renal pathological changes in cinnabar-treated rats included vacuolization of tubular cells, formation of protein casts, infiltration of inflammatory cells, and increase in the number of apoptotic tubular cells. In comparison to the control group, expression of FasL, Fas, TNF-α, TRAIL, activin A, and adiponectin was upregulated in the cinnabar-treated group. Collectively, our results suggest that prolonged use of cinnabar results in kidney damage due to accumulation of mercury and that the underlying mechanism involves apoptosis of tubular cells via a death receptor-mediated pathway.

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Copyright © 2015 Ying Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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