Genetic Variations in ABCG2 Gene Predict Breast Carcinoma Susceptibility and Clinical Outcomes after Treatment with Anthracycline-Based Chemotherapy

Figure 1

Electrophoretic patterns and DNA sequencing identification for ABCG2 G34A, C421A polymorphisms. (a1) Representative PCR-RFLP assay for different genotypes containing ABCG2 G34A polymorphism site: GG genotype (lanes 1, 3, 6, 8, and 10), GA genotype (lanes 4, 5, 7, and 9), AA genotype (lane 2), and D2000DNA ladder marker (lane M); (a2) G34A GG genotype; (a3) G34A GA genotype; (a4) G34A AA genotype. (b1) Representative PCR-RFLP assay for different genotypes containing ABCG2 C421A polymorphism site: CC genotype (lane 3), CA genotype (lanes 1, 4, and 5), AA genotype (lanes 2 and 6), and D2000DNA ladder marker (lane M); (b2) C421A CC genotype; (b3) C421A CA genotype; (b4) C421A AA genotype.