The Histone Acetyltransferase Gcn5 Regulates ncRNA-ICR1 and FLO11 Expression during Pseudohyphal Development in Saccharomyces cerevisiae

Figure 3

The transcription factor Gcn4 is recruited to the FLO11 promoter during the course of 3AT induction. (a) A schematic representation of the amplicon locations at the upstream region of FLO11 promoter with primer sets for chromatin immunoprecipitation analysis (ChIP-qPCR). +1 indicates the transcription start site of FLO11. Cells from haploids (b) and diploids (c) were collected at the indicated times to measure the chromatin association of Gcn4 at the FLO11 promoter region (NR1~NR8, noncoding RNA) during 3AT induction. Association of Gcn4-Flag (a chromosome-integrated copy of GCN4 tagged with a Flag epitope) at the NR sites was detected by ChIP and anti-Flag antibody. Data are mean ± SD from three biological repeats and normalized to input (IP/INP). The fold changes of amplified genomic DNA relative to ACT1 are indicated (internal control of qPCR).

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