Analysis of the Isomerase and Chaperone-Like Activities of an Amebic PDI (EhPDI)

Table 2

In vivo isomerase activity of EhPDI enzyme variants.

Enzyme variant	Acid phosphatase (AppA)^∗ activity in Miller units (%)
EhPDISS/SS	732 ± 74 (−1)
EhPDISS/CC	1086 ± 136 (7)
EhPDICC/SS	1646 ± 230 (20)

The data was expressed as ± standard error ( = 6).
Under the background of ΔC/pBAD-AppA, normalization was performed considering the mean activity of pBPelB-EhPDI as maximal ( = 5151) and pBluescript SK- as minimal ( = 780). The percentage (%) was calculated as [()/()] × 100, where A represents the activity of the enzyme variant.