Production by Tobacco Transplastomic Plants of Recombinant Fungal and Bacterial Cell-Wall Degrading Enzymes to Be Used for Cellulosic Biomass Saccharification

Figure 3

Northern blot analysis showing the expression of transgenes in tobacco chloroplasts. RNA isolated from untransformed control (Wt) and plastid transformed (PT) was separated on formaldehyde-agarose gels, blotted on to the Hybond-N+ membrane, and hybridized with gene specific probes. For the loading control, the same blots were hybridized again with 16S rRNA probe (lower panel): (1) Nt. Vlp2, (2) Nt. TF6A, (3) Nt. Pga2, (4) Nt. EndoV, (5) Nt. Cel6, (6) Nt. Cel7, (7) Nt. CelK1, and (8) Nt. Cel3. The red asterisks indicate the putative longer transcript initiated by the upstream rbcL promoter element.