Qat Chewing and Periodontal Pathogens in Health and Disease: Further Evidence for a Prebiotic-Like Effect

Table 1

Sequences of the primers and probes used in the quantitative PCR assays [20].

Test species	Sequences 5′-3′	Target gene	Product size
Total bacteria	F-primer: AAACTCAAAGGAATTGACGGGG; R-primer: TTGCGCTCGTTGCGGGACT; Probe: FAM-CTGTCGTCAGCTCGTGTCGTGA-BHQ	16S rRNA	205 bp
P. gingivalis	F-primer: ACGAATCAAAGGTGGCTAAGTT; R-primer: TTAGTCGCATTTTCGGCTGAT; Probe: FAM-CCTGCTGTTCTCCATTATAAACCATTACGG-BHQ	fimA	85 bp
T. forsythia	F-primer: GATAGGCTTAACACATGCAAGTC; R-primer: GTTGCGGGCAGGTTACATAC; Probe: FAM-TTACTCACCCGTGCGCCGGTCG-BHQ	16S rRNA	99 bp
T. denticola	F-primer: GGGCGGCTTGAAATAATRATG; R-primer: CTCCCTTACCGTTCGACTTG; Probe: FAM-CAGCGTTCGTTCTGAGCCAGGATCA-BHQ	16S rRNA	92 bp
P. micra	F-primer: TGAGCAACCTACCTTACACAG; R-primer: GCCCTTCTTACACCGATAAATC; Probe: FAM-ACCGCATGAGACCACAGAATCGCA-BHQ	16S rRNA	112 bp
Oral  Synergistetes	F-primer: GGAGTACGGTCGCAAGATTG; R-primer: GTAAGGTTCTTCGGTTTGCATC; Probe: FAM-ACAAGCGGTGGAGCACGTGGTTTAAT-BHQ	16S rRNA	98 bp
Filifactor alocis	F-primer: ACCCTCAAGTTGCCAAAATTATTAT; R-primer: TACTCCCTTTCTTCTGGTTAAATCT; Probe: FAM-TCGCTCTTTTTGCCGCCTCTCTTGC-BHQ	16S rRNA	101 bp
Oral TM7s	F-primer: GCTCGTGTCGTGAGATGTTT; R-primer: ATCCCCTCCTTCCTCCCCG; Probe: FAM-TAAGTCCATCAACGAGCGCAACCCTT-BHQ	16S rRNA	107 bp

The primers/probe set covers Fretibacterium fastidiosum, Fretibacterium sp. oral taxons 358, 359, 360, 361, 362, 452, and 453, Jonquetella anthropi, and Pyramidobacter piscolens.
The primers/probe set covers TM7 oral taxons 347, 348, 350, 351, 355, 356 (I025), and 437.