Decreased Splenic T-Lymphocytes in Apolipoprotein M Gene Deficient Mice

Figure 2

Distribution of splenic T-lymphocyte and its subpopulations in apoM^−/− and apoM^+/+ mice with or without administration of LPS. (a) Total CD3⁺ T-lymphocytes were slightly lower in apoM^−/− than in apoM^+/+ mice. (b) The proportion of CD4⁺ T-lymphocytes was obviously lower in apoM^−/− mice than that in apoM^+/+ mice before LPS treatment. After administration of LPS, the CD4⁺ T-lymphocytes were dramatically decreased in the apoM^−/− mice, whereas CD4⁺ T-lymphocytes were only moderately decreased. (c) The ratio of CD4⁺/CD8⁺ was also obviously decreased in apoM^−/− mice compared to that in apoM^+/+ mice after LPS treatment. (d) Representative FACS plots of splenic CD4⁺ and CD8⁺ T-lymphocytes in the apoM^+/+ and apoM^−/− mice ( 5~6, one-way ANOVA followed by Dunnett’s test, , , and versus apoM^+/+ or apoM^−/− control; Mann-Whitney test, and ).

(a)

(b)

(c)

(d)