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BioMed Research International
Volume 2015, Article ID 293570, 11 pages
Research Article

Regenerating Salivary Glands in the Microenvironment of Induced Pluripotent Stem Cells

1Department of Oral-Facial Disorders, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, Japan
2Division of Orthopedic Surgery, The Children’s Hospital of Philadelphia Research Institute, Philadelphia, PA 19104, USA
3Clinical Laboratory, Osaka University Dental Hospital, Suita, Osaka 565-0871, Japan
4Division of Molecular and Regenerative Prosthodontics, Graduate School of Dentistry, Tohoku University, Sendai, Miyagi 980-8575, Japan

Received 5 March 2015; Accepted 4 June 2015

Academic Editor: Chung-Liang Chien

Copyright © 2015 Hitomi Ono et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This report describes our initial attempt to regenerate salivary glands using induced pluripotent stem (iPS) cells in vivo and in vitro. Glandular tissues that were similar to the adult submandibular glands (SMGs) and sublingual glands could be partially produced by the transplantation of iPS cells into mouse salivary glands. However, the tumorigenicity of iPS cells has not been resolved yet. It is well known that stem cells affect their microenvironment, known as a stem cell niche. We focused on the niche and the interaction between iPS cells and salivary gland cells in our study on salivary gland regeneration. Coculture of embryonic SMG cells and iPS cells have better-developed epithelial structures and fewer undifferentiated specific markers than monoculture of embryonic SMG cells in vitro. These results suggest that iPS cells have a potential ability to accelerate differentiation for salivary gland development and regeneration.