Regenerating Salivary Glands in the Microenvironment of Induced Pluripotent Stem Cells

Figure 6

Distribution of Sox2 in developing SMG and in regenerated SMGs (SG and iSG). Sox2 is expressed in the cytoplasm of both epithelial cells and mesenchymal cells in E13.5, E15.5, and E17.5 developing SMGs and is gradually decreased in later embryonic stages. Sox2 is expressed in the nucleus of epithelial cells in both postnatal and adult SMGs. Sox2 (cyan) and E-cadherin (red) (a). Scale bar, 25 µm. Sox2 expression in the cytoplasm of both epithelial cells and mesenchymal cells in iSG (20% iSG) is less than that in SG. The nucleus of GFP-iPS cells highly expressed Sox2 as a positive control. Sox2 (cyan), GFP (green), and E-cadherin (red) (b). Scale bar, 25 µm.