Research Article
Nanoparticle Labeling of Bone Marrow-Derived Rat Mesenchymal Stem Cells: Their Use in Differentiation and Tracking
Table 1
RT-PCR conditions for each primer.
| | Beta-actin | Forward
Reverse | 5′-CTGGCCTCACTGTCCACCTT-3′
5′-GGGCCGGACTCATCGTACT-3′ | 65 bp |
| | CD90 | Forward
Reverse | 5′-CCAGTCATCAGCATCACTCT-3′
5′-AGCTTGTCTCTGATCACATT-3′ | 374 bp |
| | CD34 | Forward
Reverse | 5′-TGTCTGCTCCTTGAATCT-3′
5′-CCTGTGGGACTCCAACT-3′ | 281 bp |
| | CD71 | Forward
Reverse | 5′-ATGGTTCGTACAGCAGCAGA-3′
5′-CGAGCAGAATACAGCCATTG-3′ | 182 bp |
| | CD29 | Forward
Reverse | 5′-ACTTCAGACTTCCGCATTGG-3′
5′-GCTGCTGACCAACAAGTTCA-3′ | 190 bp |
| | CD45 | Forward
Reverse | 5′-ATGTTATTGGGAGGGTGCAA-3′
5′-AAAATGTAACGCGCTTCAGG-3′ | 175 bp |
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