Prokineticin 2 Upregulation in the Peripheral Nervous System Has a Major Role in Triggering and Maintaining Neuropathic Pain in the Chronic Constriction Injury Model

Figure 7

Representative images of CCI-induced upregulation of PROK2 in the longitudinally sliced sciatic nerve proximal and distal to the lesion. At 10 dpi a dramatic increase of PROK2 signal (green, a and c) in fibres and in GFAP+ structures (yellow) was evident both proximal and distal to the lesion. The PROK2 signal was dramatically reduced in the nerve from CCI-PC1 mice (green, b and d). Scale bar: 30 μm. High-magnification images showed macrophages that infiltrate the nerve distal to the lesion (scale bar: 10 μm). (e) Double immunofluorescence labelling for PROK2 (green) and CD11b (red) showing that in the CCI-saline mice the infiltrating macrophages contain PROK2 (yellow). (f) PROK2 signal was absent in macrophages infiltrating the nerve from CCI-PC1 mice. (e^′, f^′) CD11b (red), and (e^′′, f^′′) PROK2 (green) shown in single channels. Cell nuclei were counterstained with DAPI (blue fluorescence).