Expression of Heterologous Cellulases in Thermotoga sp. Strain RQ2

Table 2

Strains and vectors used in this study.

Strain or plasmid	Description	Reference
E. coli
DH5α	F^−  endA1  hsdR17  (rk^−,  mk^+)  supE44  thi-1  λ^−  recA1  gyrA96   relA1  deoR  Δ(lacZYA-argF)-U169ϕ80dlacZΔM15	[24]
Thermotoga
T. sp. strain RQ2	Isolated from geothermally heated sea sediment, Ribeira Quente, Săo Miguel, Azores.	[25]
T. maritima MSB8	The first type strain of Thermotoga, isolated from geothermally heated sea sediments, Porto di Levante, Vulcano, Italy.	[25]
Caldicellulosiruptor
C. saccharolyticus DSM 8903	Isolated from wood in the flow of geothermal spring, Taupo, New Zealand.	[14]
Plasmids
pDH10	Thermotoga-E. coli shuttle vector; Ap^r,	[23]
pDH26	pDH10-derived, with BsaI site erased; Ap^r, Kan^r	This study
pDH27	pDH26-derived, with NdeI site erased; Ap^r, Kan^r	This study
pHX01	pDH27-derived, having lacZ residual sequence removed; Ap^r, Kan^r	This study
pHX02.1	Promoter and signal peptide region of TM1840 (amyA) were inserted into the NotI-SacI sites of pHX01; Ap^r, Kan^r	This study
pHX02	Coding region (without the signal peptide) of Csac_1078 (celB) was inserted into BsaI-SacI sites of pHX02.1; Ap^r, Kan^r	This study
pHX04.1	Promoter and signal peptide region of TM0070 (xynB) were inserted into the NotI-SacI sites of pHX01; Ap^r, Kan^r	This study
pHX04	Coding region (without the signal peptide) of Csac_1078 (celB) was inserted into the BsaI-SacI sites of pHX04.1; Ap^r, Kan^r	This study
pHX07.1	Promoter and signal peptide region of TM0070 (xynB) were inserted into the XhoI-PstI sites of pHX01; Ap^r, Kan^r	This study
pHX07	Coding region (without the signal peptide) of Csac_1076 (celA) was inserted into the BsaI-PstI sites of pHX07.1; Ap^r, Kan^r	This study

Ap: ampicillin; Kan: kanamycin.