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BioMed Research International
Volume 2015, Article ID 304753, 13 pages
Research Article

A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21Waf1/CIP1 Expression

1Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1095 Jie Fang Avenue, Wuhan, Hubei 430030, China
2Institute of Urology of Hubei Province, No. 1095 Jie Fang Avenue, Wuhan, Hubei 430030, China

Received 3 January 2015; Revised 9 March 2015; Accepted 11 March 2015

Academic Editor: Jiing-Kuan Yee

Copyright © 2015 Chenghe Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Table S1: All dsRNAs used in present study werechemically synthesized with dTdT 3'overhangs. The dsP21-397 complementary to the p21 promoter at sequences position -397 relative to the transcription starting site was used to activate p21 expression. The control dsRNA (dsControl) was designed to lack significant homology to all known human genes' sequences. The dsRNA (siP21) was used to silence p21 expression by targeting p21 mRNA 3' untranslated regions.

Supplementary Table S2: The primes were used to detect p21 and GAPDH mRNA relative expression levels in BC cell lines by real-time PCR.

Figure S1: In order to compare the activating activities between miR-1180-5p and dsP21-397, we transfected T24 and EJ cells with miR-1180-5p or dsP21-397 at 0nM, 25nM, 50nM and 100nM, respectively. 72 h later, p21 mRNA was determined by real-time PCR and the miR-1180-5p induced p21 mRNA expression levels were normalized to dsP21-397 groups at the same concentrations. As shown in Figure S1A and S1B, the p21 expression was not significantly different between miR-1180-5p and dsP21-397 at the same transfecting concentrations in T24 and EJ cells.

  1. Supplementary Material