Research Article
Characterization of Multidrug Resistant Extended-Spectrum Beta-Lactamase-Producing Escherichia coli among Uropathogens of Pediatrics in North of Iran
Table 1
The sequences of primers and thermal condition used in PCR amplification.
| Target genes | Primer used (5′-3′) | Thermal cycling condition | PCR product size |
| TEM | TAATCAGTGAGGCACCTATCTC GAGTATTCAACATTTCCGTGTC [18, 19] |
94°C 3 min → 35 94°C 30 sec, 45°C 45 sec, 72°C 40 sec → 72°C 7 min | 800 bp |
| CTX | TTTGCGATGTGCAGTACCAGTAA CGATATCGTTGGTGGCATA [20] | 94°C 5 min → 40 94°C 45 sec, 53.1°C 45 sec, 72°C 1 min → 72°C 7 min | 593 bp |
| VEB | CGACTTCCATTTCCCGATGC GGACTCTGCAACAAATACGC [9] | 93°C 3 min → 40 93°C 1 min, 54.9°C 1 min, 72°C 1 min → 72°C 7 min | 585 bp |
| SHV | GGTTATGCGTTATATTCGCC TTAGCGTTGCCAGTGCTC [21] | 1 cycle of 5 min at 96°C; 35 cycles of 1 min at 96°C, 1 min at 60°C, 1 min at 72°C; 1 cycle of 10 min at 72°C | 867 bp |
| GES | ATGCGCTTCATTCACGCAC CTATTTGTCCGTGCTCAGG [22] | 1 cycle of 5 min at 95°C; 30 cycles of 1 min at 95°C, 45 sec at 55°C, 1 min 30 sec at 72°C; 1 cycle of 8 min at 72°C | 846 bp |
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