Molecular PET Imaging of Cyclophosphamide Induced Apoptosis with 18F-ML-8
Table 1
Accumulation of 18F-ML-8 in Jurkat cells.
Experiment and cells
Mean ± SD % uptake/108 cells
Treatment
Inhibition
Induction of apoptosis
Control
0.126 ± 0.022
Treated
0.529 ± 0.040
Anti-Fas antibody
Inhibition of apoptosis
Control
0.106 ± 0.007
Z-VAD-FMK
Treated
0.131 ± 0.038
Anti-Fas antibody
Z-VAD-FMK
Induction of necrosis
Control
0.142 ± 0.080
Treated
0.204 ± 0.004
Freeze-thaw
Apoptosis was induced in Jurkat cells by treatment with anti-Fas, and necrotic cell death was induced by three thaw cycles. Each value is the mean ± SD % out of the three experiments, P < 0.01 compared with control group.
