Research Article

Human Umbilical Cord Mesenchymal Stem Cells Inhibit the Function of Allogeneic Activated Vγ9Vδ2 T Lymphocytes In Vitro

Figure 1

UC-MSCs inhibit γδ T cells proliferation in a dose-dependent manner. PBMCs from healthy donors were stained with CFSE (1 μM) and cocultured with, or without, UC-MSCs at the indicated (T : MSCs) ratios for 14 days. The cells were stained with different fluorescent antibodies, as described in in the method section, and the percentages of proliferative γδ T cells were determined by flow cytometry. The cells were gated on CD3+TCRγδ+ cells and their proliferation was characterized by flow cytometry, followed by quantification. Data are representative charts and histograms or expressed as the means ± SEM of each group of cells from 15 healthy donors. (a) The representative population of CD3+TCRγδ+ cells following coculture with, or without, UC-MSCs. (b) The representative histograms of proliferative CD3+TCRγδ+ cells. (c) The percentages of proliferative CD3+TCRγδ+ cells. versus the controls without coculture with UC-MSCs. UC-MSC: umbilical cord mesenchymal stem cell; PBMC: peripheral blood mononuclear cell; CFSE: 5,6-carboxyfluorescein diacetate succinimidyl ester.
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