Effects of Extrusion Pretreatment Parameters on Sweet Sorghum Bagasse Enzymatic Hydrolysis and Its Subsequent Conversion into Bioethanol
Table 1
Different sugars and ethanol concentrations from extruded sweet sorghum bagasse at 100°C, 200 rpm, and 30% moisture, fermented with Issatchenkia orientalis 20381 at 12% solids fraction after 72 h enzymatic saccharification1,2.
Compound [mg·g−1]
Issatchenkia orientalis ATCC 20381 at 12% solids loading ferementation
Issatchenkia orientalis ATCC 20381 without solids
0 h
12 h
24 h
48 h
72 h
0 h
12 h
24 h
48 h
72 h
Arabinose
4.20 ± 0.08
ND
ND
ND
ND
4.26 ± 0.07
ND
ND
ND
ND
Cellobiose
3.94 ± 0.27
12.29 ± 2.32
12.07 ± 0.06
6.30 ± 0.25
2.75 ± 0.05
3.31 ± 0.15
12.84 ± 0.36
12.92 ± 0.36
10.41 ± 0.13
9.88 ± 0.77
Galactose
3.71 ± 0.28
2.90 ± 0.12
1.51 ± 0.15
0.47 ± 0.01
0.16 ± 0.01
3.69 ± 0.06
0.27 ± 0.04
ND
ND
ND
Glucose
337.01 ± 1.10
250.78 ± 13.76
2.00 ± 0.35
1.48 ± 0.44
ND
326.92 ± 4.25
213.83 ± 5.26
11.45 ± 3.96
ND
ND
Mannose
ND
1.22 ± 0.08
2.30 ± 0.19
2.70 ± 0.03
3.09 ± 0.05
2.07 ± 0.03
1.46 ± 0.08
1.63 ± 0.17
2.01 ± 0.01
2.03 ± 0.19
Xylose
36.80 ± 0.29
27.60 ± 0.53
28.30 ± 0.53
26.61 ± 0.22
23.39 ± 2.12
32.51 ± 0.18
24.16 ± 1.03
28.45 ± 1.82
26.96 ± 1.70
22.23 ± 0.91
Total sugars
385.66 ± 6.79
294.79 ± 11.71
45.90 ± 0.48
37.56 ± 0.01
29.39 ± 2.05
372.76 ± 4.74
252.60 ± 6.77
54.44 ± 1.12
39.37 ± 1.56
31.14 ± 1.87
Ethanol
ND
21.78 ± 1.10
160.06 ± 3.10
157.89 ± 3.03
147.63 ± 1.81
ND
16.94 ± 0.49
154.34 ± 3.24
156.36 ± 1.15
127.63 ± 5.66
Free amino nitrogen3
98.65 ± 0.95
93.13 ± 4.00
49.46 ± 4.76
47.57 ± 3.24
47.30 ± 0.95
144.88 ± 0.38
138.81 ± 3.43
75.74 ± 1.52
75.45 ± 1.72
50.94 ± 3.81
The compounds are in milligrams of sugar per gram of sweet sorghum bagasse dry basis.
2The compounds not detected by the HPLC were reported with the acronym ND.
3The free amino nitrogen is in milligrams per liter.
