Research Article

CRISPR/CAS9-Mediated Genome Editing of miRNA-155 Inhibits Proinflammatory Cytokine Production by RAW264.7 Cells

Figure 1

miR-155 genome knockout strategy. (a) Gene structure of mouse SHIP1 and miR-155 targeting sequence within 3′-UTR. The seed sequences of miR-155 were in red and the PAM sequence within miR-155 genomic DNA was in green. gRNA sequence was highlighted in blue. The predicted CAS9 cutting site was indicated with a red triangle. (b) Structural illustration of lentiCRISPR vector. (c) T7 endonuclease I assay for CRISPR efficiency. Bands representing indel formation were indicated with two light blue dots.
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