MiR-183 Regulates ITGB1P Expression and Promotes Invasion of Endometrial Stromal Cells

Figure 2

Confirmation of microarray data with Western blotting analyses. The endometrial stromal cells transfected with miR-183-lentivirus (low dose or high dose), In-miR-183-lentivirus (low dose or high dose), and GFP-lentivirus (NC, normal control) were used to examine the expression alterations of integrin β1 (a) and AMIGO2 (b). β-actin served as protein loading control. Compared with the control group, integrin β1 expression in the miR-183-overexpression group was significantly decreased, whereas it increased sharply in cells with low miR-183 expression. AMIGO2 was largely not regulated by miR-183. when compared to the negative control. Error bars represent ± SEM.

(a)

(b)