Neem (Azadirachta indica A. Juss) Oil to Tackle Enteropathogenic Escherichia coli
Table 5
Bacterial growth reduction (%) at 24 h in liquid medium with different concentrations of NSO, using as reference the control treatment (without NSO).
E. coli isolates
Percent growth reduction zone (%)
NSO (100 µL)
NSO (10 µL)
NSO (1 µL)
NSO (0.1 µL)
(1) FLC 1056
39.25 ± 1.43 c
35.61 ± 1.00 b
21.67 ± 1.33 a
11.31 ± 2.08 a
(2) FLC 1247
25.51 ± 1.15 c
24.70 ± 1.00 b
21.88 ± 1.33 a
11.86 ± 1.00 a
(3) FLC 1059
31.51 ± 1.15 c
25.70 ± 1.00 b
24.58 ± 1.33 a
14.86 ± 1.00 a
(4) FLC 1243
38.90 ± 1.00 d
28.79 ± 1.00 c
29.40 ± 0.00 b
16.68 ± 1.20 a
(5) FLC 1048
25.60 ± 1.53 d
23.73 ± 2.08 b
23.69 ± 2.00 b
12.83 ± 1.73 a
(6) FLC 1167
23.71 ± 1.00 b
21.61 ± 0.58 a
21.27 ± 0.00 a
11.18 ± 0.89 a
(7) FLC 1249
29.65 ± 1.53 b
28.61 ± 1.00 a
28.77 ± 1.33 a
18.51 ± 2.08 a
(8) FLC 1055
34.51 ± 1.15 c
29.70 ± 1.00 c
24.38 ± 1.33 a
14.86 ± 1.00 a
(9) FLC 1054
31.51 ± 1.15 d
29.70 ± 1.00 c
25. 78 ± 1.33 a
14.86 ± 1.00 a
(10) FLC 1085
39.90 ± 1.00 c
28.79 ± 1.00 b
28.10 ± 0.00 b
15.58 ± 1.20 a
(11) FLC 1244
35.70 ± 1.53 d
28.73 ± 2.08 b
28.59 ± 2.00 b
12.63 ± 1.73 a
(12) FLC 1165
39.71 ± 1.00 c
29.61 ± 0.58 c
29.17 ± 0.00 b
16.48 ± 0.89 a
(13) FLC 1086
39.55 ± 1.53 c
28.61 ± 1.00 c
27.87 ± 1.33 b
12.21 ± 2.08 a
(14) FLC 1053
34.51 ± 1.15 c
89.70 ± 1.00 c
27.58 ± 1.33 b
14.86 ± 1.00 a
(15) FLC 1095
31.51 ± 1.15 c
29.70 ± 1.00 b
25.18 ± 1.33 a
14.86 ± 1.00 a
(16) FLC 1219
38.95 ± 1.00 b
28.79 ± 1.00 b
28.10 ± 0.00 b
16.68 ± 1.20 a
(17) FLC 1235
36.70 ± 1.53 d
27.73 ± 2.08 c
27.79 ± 2.00 b
12.83 ± 1.73 a
(18) DSM8696
39.71 ± 1.00 c
29.61 ± 0.58 c
29.67 ± 0.00 b
17.58 ± 0.89 a
(19) DSM9025
39.65 ± 1.53 c
28.61 ± 1.00 c
26.17 ± 1.33 b
18.10 ± 2.08 a
(20) DSM10973
34.51 ± 1.15 c
29.70 ± 1.00 c
23.28 ± 1.33 b
14.86 ± 1.00 a
(21) ATCC33559
31.51 ± 1.15 d
29.70 ± 1.00 c
21.58 ± 1.33 b
14.86 ± 1.00 a
(22) CVVI E210
88.90 ± 1.00 c
88.79 ± 1.00 c
69.20 ± 0.00 b
14.78 ± 1.20 a
(23) CVVI E173
36.50 ± 1.53 d
27.73 ± 2.08 c
27.99 ± 2.00 b
22.83 ± 1.73 a
(24) CVVI E126
89.81 ± 1.00 c
89.61 ± 0.58 c
69.37 ± 0.00 b
44.58 ± 0.89 a
(25) CVVI E16
38.55 ± 1.53 c
28.61 ± 1.00 c
27.57 ± 1.33 b
11.51 ± 2.08 a
(26) CVVI E320
38.51 ± 1.15 c
29.70 ± 1.00 c
27.18 ± 1.33 b
14.86 ± 1.00 a
(27) CVVI E130
30.71 ± 1.15 c
29.70 ± 1.00 c
27.68 ± 1.33 a
14.46 ± 1.00 a
(28) CVVI E48
38.60 ± 1.00 c
28.79 ± 1.00 c
29.20 ± 0.00 a
16.68 ± 1.20 a
(29) CVVI KH10
99.60 ± 1.53 d
81.73 ± 2.08 c
68.39 ± 2.00 b
62.33 ± 1.73 a
(30) CVVI K10B
89.81 ± 1.00 c
89.61 ± 0.58 c
69.37 ± 0.00 b
60.58 ± 0.89 a
(31) CVVI E298
39.75 ± 1.53 c
28.61 ± 1.00 c
27.57 ± 1.33 b
20.11 ± 2.08 a
(32) CVVI E273
34.51 ± 1.15 c
29.70 ± 1.00 c
26.58 ± 1.33 b
22.86 ± 1.00 a
(33) CVVI K436
31.85 ± 1.15 d
29.70 ± 1.00 c
27.18 ± 1.33 b
15.86 ± 1.00 a
(34) CVVI E98
37.93 ± 1.00 c
28.79 ± 1.00 c
29.50 ± 0.00 b
16.68 ± 1.20 a
(35) CVVI E77
33.69 ± 1.53 d
21.73 ± 2.08 c
27.29 ± 2.00 b
19.83 ± 1.73 a
(36) CVVI E148
39.71 ± 1.00 c
29.61 ± 0.58 c
29.57 ± 0.00 b
22.58 ± 0.89 a
(37) CVVI E10
89.65 ± 1.53 c
88.61 ± 1.00 c
61.67 ± 1.33 b
50.79 ± 2.08 a
(38) CVVI E215
34.51 ± 1.15 c
29.70 ± 1.00 c
17.58 ± 1.33 b
21.86 ± 1.00 a
(39) NLK99/F5
91.51 ± 1.15 d
89.70 ± 1.00 c
67.68 ± 1.33 b
61.86 ± 1.00 a
(40) NL12B/F5
88.90 ± 1.00 c
88.79 ± 1.00 c
69.60 ± 0.00 b
63.68 ± 1.20 a
(41) NLK99-1*
97.70 ± 1.53 d
81.73 ± 2.08 c
68.69 ± 2.00 b
62.83 ± 1.73 a
(42) NLK99-3*
89.71 ± 1.00 c
79.61 ± 0.58 c
69.57 ± 0.00 b
67.58 ± 0.89 a
(43) NLK99-5*
89.65 ± 1.53 c
78.61 ± 1.00 c
67.67 ± 1.33 b
50.81 ± 2.08 a
(44) NLK99-7*
84.51 ± 1.15 c
79.70 ± 1.00 c
67.58 ± 1.33 b
44.86 ± 1.00 a
(45) NLK99-9*
91.51 ± 1.15 c
79.70 ± 1.00 c
67.28 ± 1.13 b
64.86 ± 1.00 a
(46) NLK99-11*
88.90 ± 1.00 c
78.79 ± 1.00 c
69.60 ± 0.00 b
66.68 ± 1.20 a
(47) NLK99-15*
99.70 ± 1.53 d
91.63 ± 0.28 c
68.69 ± 2.00 b
62.83 ± 1.73 a
(48) NLK99-19*
89.71 ± 1.00 c
79.61 ± 0.58 c
69.57 ± 0.00 b
67.58 ± 0.89 a
Three plates for each bacterium were considered. The experiment was repeated twice. Values are given as mean ± S.D. Values in a row followed by different lowercased letters are significantly different at .
