Lunasin Inhibits Cell Proliferation via Apoptosis and Reduces the Production of Proinflammatory Cytokines in Cultured Rheumatoid Arthritis Synovial Fibroblasts

Table 1

Primer sequences and reaction conditions of RT-PCR.

Genes	Primers	Sequences (5′-3′)	Annealing temperature (°C)	Cycle number
IL-6	Forward	GGCTGCTTCTGGTGATGG	55	30
	Reverse	AGAGATTTTGCCGAGGATGTA
IL-8	Forward	GCCAAGGAGTGCTAAAGAACTTAGA	58	30
	Reverse	ATTTCTGTGTTGGCGCAGTGT
MMP-1	Forward	AGGGTCAAGCAGACATCA	56	30
	Reverse	CAGAAGGGCAAGCATTAG
MMP-3	Forward	CCTGCTTTGTCCTTTGATGC	55	30
	Reverse	TGAGTCAATCCCTGGAAAGTC
GAPDH	Forward	CAAGGTCATCCATGACAACTTTG	56	25
	Reverse	GTCCACCACCCTG TTGCTGTAG