Research Article

The Differential Proteome of the Probiotic Lactobacillus acidophilus NCFM Grown on the Potential Prebiotic Cellobiose Shows Upregulation of Two β-Glycoside Hydrolases

Figure 3

(a) Schematic presentation of cellobiose entry and hydrolysis by L. acidophilus NCFM. Superscript letters and indicate upregulation by transcriptomics [19] or increase in abundance by proteomics, respectively (LBA0725: PTS II, LBA0726: phospho-β-galactosidase II, LBA0874: phospho-β-galactosidase I, LBA0876: PTS IIC, LBA0877: PTS IIA, LBA0879: PTS IIC, LBA0881: phospho-β-glucosidase, LBA0884: PTS IIC LBA0726: phospho-β-galactosidase II, and LBA0885: β-glucosidase). (b) Schematic presentation of gene clusters encoding glycoside hydrolases (LBA0726: phospho-β-galactosidase II, LBA0874: phospho-β-galactosidase I, LBA0881: phospho-β-glucosidase, and LBA0885: β-glucosidase), shown as light grey arrows and PTSs (LBA0725: PTS II, LBA0876: PTS IIC, LBA0877: PTS IIA, LBA0879: PTS IIC, and LBA0884: PTS IIC), shown as dark grey arrows, predicted to be cellobiose specific. Transcription antiterminator (LBA0724), hypothetical proteins (LBA0878, LBA0880, and LBA0883), and transcriptional regulators (LBA0875, LBA0882, and LBA0886) are shown as white arrows.
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