The Differential Proteome of the Probiotic Lactobacillus acidophilus NCFM Grown on the Potential Prebiotic Cellobiose Shows Upregulation of Two β-Glycoside Hydrolases

Table 1

Differentially abundant proteins of Lactobacillus  acidophilus NCFM grown on cellobiose compared to growth on glucose shown for selected proteins having a role in carbohydrate metabolism. Differential abundance was based on Progenesis SameSpots analyses of 2D images (≥ 1.5-fold spot volume ratio change; ANOVA and false discovery rate ). A Mascot score of ≥ 80 () was used to confirm proteins identified by peptide mass fingerprint and should have a minimum of six matched peptides. Proteins are listed according to their fold change. All identified proteins including information regarding score, ANOVA, sequence coverage, peptide search and identification, MW, and pI are available in supplementary material.

Spot number	Fold change	Accession number	Protein name	Localization^a	Functional role^b
^*102	−7.8	gi∣58337790	Two-component system regulator	C	RF
16	+7.4	gi∣58337043	Phospho-beta-galactosidase II	C	EM
18	+7.0	gi∣58337043	Phospho-beta-galactosidase II	C	EM
92	−3.6	gi∣58337251	2′,3′-Cyclic-nucleotide 2′-phosphodiesterase	E	PPNN
13	+3.5	gi∣58337008	Phosphoglucomutase	C	EM
60	+2.6	gi∣58337021	Triosephosphate isomerase	C	EM
37	−2.5	gi∣58337019	Glyceraldehyde-3P dehydrogenase	C	EM
17	+2.4	gi∣58337186	Phospho-beta-glucosidase	C	EM
47	+2.4	gi∣58336768	Catabolite control protein A	C	RF
122	+2.2	gi∣58337323	Putative surface layer protein	CW	CE
123	+2.0	gi∣58337019	Glyceraldehyde-3P dehydrogenase	C	EM
99	−1.9	gi∣58337088	F0F1 ATP synthase subunit alpha	C	EM
59	−1.8	gi∣58337021	Triosephosphate isomerase	C	EM
29	−1.7	gi∣58337020	Phosphoglycerate kinase	C	EM
106	−1.7	gi∣58337021	Triosephosphate isomerase	C	EM
28	−1.6	gi∣58337020	Phosphoglycerate kinase	C	EM
30	−1.6	gi∣58337020	Phosphoglycerate kinase	C	EM
31	−1.6	gi∣58337020	Phosphoglycerate kinase	C	EM
38	−1.6	gi∣58337019	Glyceraldehyde-3P dehydrogenase	C	EM
41	−1.6	gi∣58337089	F0F1 ATP synthase subunit gamma	C	EM
36	+1.5	gi∣58337019	Glyceraldehyde-3P dehydrogenase	C	EM
70	−1.5	gi∣58337019	Glyceraldehyde-3P dehydrogenase	C	EM
107	−1.5	gi∣58337021	Triosephosphate isomerase	C	EM

Localization: C, cytoplasmic; CW, cell wall; E, extracellular.
^bFunctional role: CE, cell envelope; EM, energy metabolism; PPNN, purines, pyrimidines, nucleosides, and nucleotides; RF, regulatory functions.
^*Protein identification by MS/MS was confirmed by a Mascot score of ≥ 40 () for each peptide.