Duck RIG-I CARD Domain Induces the Chicken IFN-β by Activating NF-κB

Table 1

Primer information for vector construction and RT-qPCR.

Name of primer	Sequences (5′→3′)	Annealing temperature (°C)	Note
RIG-I-Full-F; RIG-I-Full-R	CCGGGTACCATGACGGCGGACGAGAAGCGGAG; TGCTCTAGACTAatgatgatgatgatgatgAAATGGTGGGTACAAGTTGGAC	68	CDS amplification
RIG-I-Full-F; RIG-I-N-R	CCGGGTACCATGACGGCGGACGAGAAGCGGAG; TGCTCTAGACTAatgatgatgatgatgatgCTTTGTTTCATAGACAGGTGGAGGTTTGC	68	N-terminal amplification
RIG-I-C-F; RIG-I-Full-R	CCGGGTACCATGGCCAAAGATGTTGACAGTGAAATGA; TGCTCTAGACTAatgatgatgatgatgatgAAATGGTGGGTACAAGTTGGAC	68	C-terminal amplification

Note. The underlined italics indicate the enzyme cutting site, the lowercase italics indicate the 6*His tag sequence, and the bold letters indicate additional termination and initiation codons.