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BioMed Research International
Volume 2015, Article ID 352029, 8 pages
http://dx.doi.org/10.1155/2015/352029
Research Article

Contribution of Avian Salmonella enterica Isolates to Human Salmonellosis Cases in Constantine (Algeria)

1Laboratory of Health Management and Animal Production, Veterinary Sciences Department, Constantine Mentouri University, Constantine, Algeria
2Alfort National Veterinary School, Paris-Est University, 94704 Maisons-Alfort Cedex, France
3Antimicrobial Resistance Unit, Maisons-Alfort Laboratory for Food Safety, ANSES, Paris-Est University, 94701 Maisons-Alfort Cedex, France
4Hygiene and Quality of Poultry and Pig Products Unit, Anses Ploufragan Laboratory, European University of Brittany, 22440 Ploufragan, France
5Service de Microbiologie-Bactériologie, C.H.U. Ibn Badis, Constantine, Algeria

Received 28 November 2014; Revised 16 February 2015; Accepted 22 February 2015

Academic Editor: Avelino Alvarez-Ordóñez

Copyright © 2015 Rachid Elgroud et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

An epidemiological investigation was carried out on one hundred Salmonella isolates from broiler farms, slaughterhouses, and human patients in the Constantine region of Algeria, in order to explore the contribution of avian strains to human salmonellosis cases in this region over the same period of time. The isolates were characterized by phenotypic as well as genotypic methods. A large variety of antimicrobial resistance profiles was found among human isolates, while only seven profiles were found among avian isolates. Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR), Insertion Sequence 200-PCR (IS200-PCR), and Pulsed Field Gel Electrophoresis (PFGE) resulted in the allocation of the isolates to 16, 20, and 34 different profiles, respectively. The 3 genotyping methods led to complementary results by underlining the clonality of some serovars with the diffusion and persistence of a single clone in the Constantine area as well as stressing the polymorphism present in isolates belonging to other serovars, indicating the diversity of potential reservoirs of nontyphoidal Salmonella. Altogether, our results seem to indicate that nontyphoidal avian Salmonella may play an important role in human salmonellosis in the Constantine region.