Research Article
Dose Response of Endotoxin on Hepatocyte and Muscle Mitochondrial Respiration In Vitro
Figure 4
Tracings from the OROBOROS-high-resolution respirometry of permeabilized cells. Legend: the upper tracing represents a cell sample incubated with placebo (control) for 24 hours; the lower tracing represents a cell sample at the same passage number incubated with 1 μg/mL LPS for 24 hours. The experiments were recorded simultaneously. The blue line represents oxygen concentration; the red line represents the oxygen flow (slope of oxgygen concentration). GM: Glutamate + malate, ADP: adenosine diphosphate, ROT: rotenone, SUC: succinate, AA: antimycin A, ASC TMPD: ascorbate + N,N,N′,N′-tetramethyl-p-phenylenediamine, NaN3: sodium azide, and S3: state 3. State 4 cannot be measured due to the saturating concentration of ADP during the experiment.