Dose Response of Endotoxin on Hepatocyte and Muscle Mitochondrial Respiration In Vitro

Figure 5

Basal respiration of intact cells. Primary human hepatocytes’ basal oxygen consumption, in the presence of oligomycin (leak respiration), and FCCP, respectively, in LPS treated and control cells ((a); each). The uncoupled respiratory control ratio (uRCR) was calculated as the ratio between the oxygen consumption rate in the presence of FCCP and the rate in the presence of oligomycin (b) and the coupling efficiency was calculated as the ratio between oligomycin-sensitive and basal respiration rates (c). Data represented as box and whiskers. Statistical significance between samples was assessed using a Wilcoxon test. c: control; lps: 1 μg/mL LPS for 8 hours.

(a)

(b)

(c)