Dose Response of Endotoxin on Hepatocyte and Muscle Mitochondrial Respiration In Vitro

Table 1

Effects of LPS exposure on cellular respiration and cell viability of cultured, digitonin-permeabilized human hepatoma cells (HepG2) for various time intervals and at different concentrations. For cellular respiration, all values are expressed as pmol O₂/(sec ∗ Mill. Cells); ^∗∗; = 10 each (LPS treated and control cells); data are expressed as median (IQR). Friedman test followed by Dunn's correction of mutliple comparisons was used to detect differences between doses of LPS at each time point. If significant, a Wilcoxon test was used to compare the difference compared to the corresponding control group. Wilcoxon of complex II 24 hours 1 g LPS/mL versus control: = 0.002; Wilcoxon of complex IV 24 hours 1 g LPS/mL versus control: = 0.004.

HepG2 permeabilized		Control	0.1 g LPS/mL	1 g LPS/mL	10 g LPS/mL	Friedman
Complex I	4 h	99 (86–112)	107 (88–115)	105 (101–111)	106 (98–119)	0.430
	8 h	90 (78–99)	89 (77–105)	89 (82–96)	88 (76–95)	0.392
	16 h	95 (68–101)	96 (83–106)	95 (84–101)	89 (81–99)	0.472
	24 h	96 (82–110)	105 (92–111)	87 (84–97)	91 (77–110)	0.339
Complex II	4 h	121 (107–126)	115 (108–134)	123 (109–127)	124 (114–130)	0.516
	8 h	126 (109–135)	117 (95–133)	131 (118–145)	125 (99–135)	0.339
	16 h	104 (81–129)	120 (91–132)	115 (95–126)	108 (95–126)	0.669
	24 h	94 (78–111)	94 (84–105)	(68–93)	84 (67–99)	0.006
Complex IV	4 h	172 (166–187)	179 (155–210)	170 (166–181)	174 (161–185)	0.669
	8 h	166 (97–200)	144 (118–166)	145 (126–168)	147 (129–173)	0.840
	16 h	139 (90–171)	127 (108–165)	123 (111–156)	122 (109–174)	0.840
	24 h	98 (90–130)	99 (84–118)	(63–93)	98 (89–133)	0.007
Viability (%)	8 h	84 (68–91)	87 (72–95)	88 (71–93)	88 (70–92)
	16 h	95 (93–96)	93 (90–94)	94 (91–95)	94 (90–95)
	24 h	94 (92–95)	92 (89–93)	94 (93–95)	93 (90–95)