Dose Response of Endotoxin on Hepatocyte and Muscle Mitochondrial Respiration In Vitro

Table 3

Time and dose course of LPS on isolated pig skeletal muscle mitochondria. Data are expressed as median (IQR). All values are in pmol O₂/(sec ∗ mg mitochondrial protein). for each experiment in LPS treated and control isolated mitochondria. ^∗Wilcoxon compared to its time-matched control sample. A Friedman test was used to compare a dose effect at each time point. If significant, a Wilcoxon test was performed to compare the difference to its time-matched control sample (^∗). Additionally, a Wilcoxon test was performed to detect differences in control samples measured 2 and 4 hours after isolation ().

Isolated mitochondria		Control	0.1 g LPS/mg	1 g LPS/mg	10 g LPS/mg	50 g LPS/mg	100 g LPS/mg	Friedman
Complex I state 3	2 h	1676 (1171–1914)	1512 (1093–1527)	1365 (1110–1790)	1396 (992–1591)	(725–1562)	(788–1443)	0.008
	4 h	1178	1250 (493–1742)	1050 (657–1548)	1207 (407–1814)	1258 (584–1543)	1081 (804–1424)	0.576
Complex I state 4	2 h	454 (306–556)	420 (267–525)	418 (259–558)	419 (198–526)	(162–413)	(171–423)	0.003
	4 h	269 (191–445)	309 (175–427)	310 (205–346)	361 (149–448)	334 (204–532)	332 (201–510)	0.846
Complex I RCR	2 h	3.3 (2.7–4.5)	3.6 (2.4–4.3)	3.3 (2.9–5.7)	3.4 (2.5–3.8)	3.4 (1.9–4.6)	4.3 (2.6–5.3)	0.670
	4 h	3.6 (3.0–4.9)	4.1 (2.8–4.2)	4.1 (2.5–4.9)	4.1 (2.3–4.6)	3.9 (2.4–4.6)	3.5 (2.4–5.0)	0.946
Complex II state 3	2 h	1753 (469–2340)	1479 (449–2046)	1042 (476–1671)	1468 (388–2430)	(444–1320)	(414–1217)	0.005
	4 h	1308	1428 (459–1680)	1181 (425–1594)	1359 (326–1495)	958 (386–1019)	754 (479–1097)	0.136
Complex II state 4	2 h	631 (398–882)	576 (363–902)	578 (413–683)	591 (323–866)	627 (300–989)	637 (293–890)	0.600
	4 h	716 (322–888)	732 (375–891)	679 (337–803)	599 (284–1086)	618 (311–923)	595 (323–656)	0.227
Complex II RCR	2 h	2.2 (1.4–2.7)	2.0 (1.2–2.6)	2.0 (1.2–2.6)	2.0 (1.2–2.5)	1.2 (1.1–1.8)	1.4 (1.2–2.1)	0.123
	4 h	1.3 (1.1–2.3)	1.9 (1.1–2.1)	1.3 (1.1–2.3)	1.3 (1.2–1.9)	1.3 (1.2–1.7)	1.4 (1.2–1.9)	0.923
Complex IV state 3	2 h	2338 (684–4335)	1762 (415–4223)	977 (690–2325)	1912 (435–3679)	1890 (379–2722)	1553 (368–2379)	0.084
	4 h	1488	1553 (399–3516)	1356 (399–2320)	1276 (422–3043)	2145 (450–2344)	979 (388–1643)	0.068