Efficient and Specific Detection of Salmonella in Food Samples Using a stn-Based Loop-Mediated Isothermal Amplification Method

Table 1

Oligonucleotide primer sequences used in stn LAMP analysis.

Primer	Primer type	Sequence (5′-3′)
F3	Forward outer (F3)	5′ ACCAGATTCAGGGAGTGAGT 3′
B3	Backward outer (B3)	5′ CGCGCACGAAATTCGTAAC 3′
FIP	Forward-inner (F1c-F2)	5′ ACCGGGTGGTAAGCGAATTGC-
		GAGGTTAACCGTCTGGAGC 3′
BIP	Backward-inner (B1c-B2)	5′ TCGGCCTCTTTGGCCATCAC-
		TGGCGAAATACTTTGCCGAG 3′
Loop F	Loop forward (LF)	5′ TGGTAAAGCCCGCGCATCTG 3′
Loop B	Loop backward (LB)	5′ GCGCCAGTTCATGCGACTCG 3′

Note: primers for LAMP were designed using the stn gene (GenBank Accession number L16014) from Salmonella enterica subsp. enterica serovar Typhimurium.