Efficient and Specific Detection of Salmonella in Food Samples Using a stn-Based Loop-Mediated Isothermal Amplification Method

Table 3

Optimization of LAMP assay for Salmonella detection by targeting the stn gene.

Conditions	The tested range	Difference	Good yield	Selected condition^a
Temperature	60–65°C	1°C	64–66°C	65°C
Time	0–60 min	5 min	15–60 min	25 min
Loop primers	0.5–1.0 M	0.1 M	0.8–1.0 M	0.8 M
Outer primers	0.1–0.5 M	0.1 M	0.2–0.5 M	0.2 M
MgSO4	4–8 mM	0.5 mM	6–7.5 mM	7 mM
dNTPs	0.8–2.4 mM	0.2 mM	1.2–1.8 mM	1.6 mM
Betaine	0.5–1.5 M	0.1 M	0.6–1.5 M	1.0 M
Bst DNA polymerase	4–12 units	1 unit	8–12 units	8 units

The final concentrations of reagents or temperature and times used in LAMP analysis.
The concentration of FIP and BIP primers are fixed at 1.6 M.