Efficient and Specific Detection of Salmonella in Food Samples Using a stn-Based Loop-Mediated Isothermal Amplification Method

Table 4

Sensitivity for LAMP detection of Salmonella in artificially contaminated food samples using DNA templates prepared by the boiling method.

Total cells per^a				Enrichment time
250 mL of food suspension	Gram of food sample	1 mL of food suspension^b	LAMP reaction^c	(0 hr)	(5 hr)
5.5 × 10^6	2.2 × 10^5	2.2 × 10^4	1.1 × 10^3	+	+
5.5 × 10^5	2.2 × 10^4	2.2 × 10^3	1.1 × 10^2	+	+
5.5 × 10^4	2.2 × 10^3	2.2 × 10^2	1.1 × 10^1	+	+
5.5 × 10^3	2.2 × 10^2	2.2 × 10	1.1	+	+
5.5 × 10^2	2.2 × 10	2.2	0	−	+
5.5 × 10^1	2.2	0.2	0	−	+

The number of cells at 0 hr (before enrichment) using 25 g of food sample in 225 mL of lactose broth.
^bOne mL of food sample was used to prepare DNA template by the boiling method. Samples were resuspended in 100 L of TE buffer and used as DNA template.
^c5 L of DNA template was used in a 25 L LAMP reaction. Loop primers were included in the reaction mixture.