Identification of Reference Genes in Human Myelomonocytic Cells for Gene Expression Studies in Altered Gravity

Figure 6

Influence of altered gravity during parabolic flight on potential reference genes. RNA expression levels after 1 g (light gray), 1.8 g (dark gray), and μg (black) conditions during the 19th DLR parabolic flight campaign. Hardware ground controls (H/W, striped) are shown for each experimental group. RNA expression levels are shown as fluorescence intensities. (a) The expression values for GAPDH, HMBS, RPLP0, TBP, and YWHAZ are displayed. (b) ALB and B4GALT6 show low but stable fluorescent intensities. GAPDH, HMBS, YWHAZ, ALB, and B4GALT6 show no significant change in RNA levels upon altered gravity for 20 sec, while RPLP0 displays μg sensitivity compared to 1 g and TBP reacts sensitively to all g conditions. Mean values of at least three measurements with standard deviations are shown. , .

(a)

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