Research Article
The Type 7 Serotonin Receptor, 5-HT7, Is Essential in the Mammary Gland for Regulation of Mammary Epithelial Structure and Function
Figure 3
Lactating HT7KO mammary glands are morphologically altered and fail to undergo morphological transition associated with milk stasis induced involution. (a) A unilateral teat-sealing experiment was performed on WT and HT7KO lactating mice at peak lactation (day 10). Sealed and unsealed glands were harvested 48 hours (day 2) after sealing (). Representative contralateral (sealed and open) hematoxylin and eosin stained sections from number 4 glands are shown. Red arrowheads point to the secretory vesicles present within the mammary epithelial cells. Green arrowhead indicates secreted materials filling the alveolar lumen. Black arrowheads point to apoptotic shed cells. (b) Morphometric data on the mammary glands with respect to (i) cell height, (ii) alveolar lumen diameters, and (iii) number of shed cells per alveolus. The data was analyzed by two-way ANOVA. , , and .
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