Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2015, Article ID 365794, 14 pages
http://dx.doi.org/10.1155/2015/365794
Review Article

Towards a Universal Approach Based on Omics Technologies for the Quality Control of Food

1FEM2 Ambiente s.r.l., P.za della Scienza 2, 20126 Milan, Italy
2ZooPlantLab, Department of Biotechnology and Biosciences, University of Milano-Bicocca, P.za della Scienza 2, 20126 Milan, Italy
3BioNMR Lab, Department of Biotechnology and Biosciences, University of Milano-Bicocca, P.za della Scienza 2, 20126 Milan, Italy

Received 29 July 2015; Accepted 19 November 2015

Academic Editor: Vasilis P. Valdramidis

Copyright © 2015 Emanuele Ferri et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In the last decades, food science has greatly developed, turning from the consideration of food as mere source of energy to a growing awareness on its importance for health and particularly in reducing the risk of diseases. Such vision led to an increasing attention towards the origin and quality of raw materials as well as their derived food products. The continuous advance in molecular biology allowed setting up efficient and universal omics tools to unequivocally identify the origin of food items and their traceability. In this review, we considered the application of a genomics approach known as DNA barcoding in characterizing the composition of foodstuffs and its traceability along the food supply chain. Moreover, metabolomics analytical strategies based on Nuclear Magnetic Resonance (NMR) and Mass Spectroscopy (MS) were discussed as they also work well in evaluating food quality. The combination of both approaches allows us to define a sort of molecular labelling of food that is easily understandable by the operators involved in the food sector: producers, distributors, and consumers. Current technologies based on digital information systems such as web platforms and smartphone apps can facilitate the adoption of such molecular labelling.

1. The Demand for Universal Analytical Tools to Characterize Foodstuffs

The globalization of the food market has led to a corresponding increase in issues concerning the authenticity and safety of imported foods. Consumers are susceptible to any form of food alteration that may occur during artisanal or industrial manufacturing processes and pay attention to food ingredients as these can influence nutritional and health conditions [13]. The consumer’s awareness in terms of food quality and safety is growing and growing and implies the search for products with exhaustive labelling reporting details about the original raw materials and with assurances about the absence of harmful chemical and microbial contaminants [46]. These topics drove the development of new analytical tools in the context of food science [7]. A relevant section of approaches was the one devoted to the screening of undesired microorganisms, often occurring in foodstuffs, to ensure human safety and preventing food spoilage and/or the spread of foodborne disease outbreaks [8, 9]. Foodborne pathogens, as well as spoilage microorganisms, can already be present in the indigenous microbiota of raw materials or colonize the final food product by contamination during manufacturing [10]; therefore, laboratory analyses must be conducted both on raw materials and transformed food items. There is a great number of microorganism taxa traditionally associated with human diseases and for which every food product should be tested in order to ensure their absence. Salmonella spp. is one of the major pathogens responsible for foodborne disease outbreaks throughout the world and S. enterica is the most frequently isolated species [11]. Other important and frequently reported foodborne pathogens belong to the genera Campylobacter, Yersinia, Shigella, Vibrio, Clostridium, Bacillus, Listeria, and Staphylococcus [12, 13]. Most of these microorganisms are not easily detectable with culture-dependent approaches, but DNA-based tests that improve their detection have been developed. Most of these are based on the simultaneous detection of a wide panel of entities by using universal DNA marker regions such as the 16s rDNA or the ITS [14, 15].

DNA-based approaches have acquired a growing importance also to respond to another consumer’s request that is the authentication of both raw materials and processed food products [1]. Such a demand arose due to different factors: (i) the globalization of the food market that caused a longer and more articulated food supply chain, where raw materials are globally exported and processed in countries different from the origin; (ii) the industrialization of manufacturing processes (e.g., fermentation, biopreservation, and functionalization [16]) that are becoming more and more complex and largely unknown to the consumers; (iii) the strong modifications to which foodstuffs are subject before being sold (e.g., slicing and powdering) that impede a correct identification of the original raw materials by the consumer; (iv) the growing occurrence of allergies and intolerances related to certain foods or components of processed foodstuffs, typical of western countries. A plethora of molecular-based tools has been developed to characterize food composition and validate food authenticity [1], most of which relying on the analysis of proteins [17] and/or DNA sequences [18]. Protein-based approaches are useful in characterizing the composition of fresh products; however, these methods can be biased by several factors such as the strong food manufacturing processes, the limited number of detectable isozymes, or the high tissue and developmental stage specificity of the markers [19]. DNA markers were definitely proven to be more informative than protein-based methods because DNA better resists industrial processes such as shredding, boiling, pressure cooking, or transformations mediated by chemical agents [20, 21]. This property allows a successful identification of animal, plant, or fungi raw materials, even when they are present at small traces. Moreover, the availability of advanced technologies and efficient commercial kits for DNA extraction permits obtaining an acceptable yield of genetic material from processed or degraded biological material [8, 22, 23].

DNA analyses in food science are based on specific genome regions used as “identity markers” easily detectable by Polymerase Chain Reaction (PCR) [18]. Discontinuous molecular markers such as Amplified fragment Length Polymorphisms (AFLPs), as well as their variants (i.e., ISSR, SSAP, and SAMPL), have been successfully used in the characterization of several food raw materials [18, 24]. Moreover, species-specific makers have been developed for the most important and traded categories of animal and plant raw materials. This is the case of Single Nucleotide Polymorphisms (SNPs) and Simple Sequence Repeats (SSRs) that are largely used because of their high level of polymorphism and high reproducibility [25]. These approaches are used both in the identification of plant cultivars [26, 27] and animal breeds [28, 29] and to prevent fraudulent commercial activities [30, 31]. However, being highly species-specific, these approaches require a deep knowledge of the genotypes of the organisms and their application is often limited to a single taxon, or to a few closely related taxa. Nowadays, producers, manufacturers, distributors, and consumers advocate the development and adoption of universal tools to assess not only the origin and traceability of raw materials and derived food products but also the inadvertent occurrence of other species (i.e., contamination) or cases of species substitution (i.e., frauds). The development of innovative food-related universal tools based on DNA analysis will be the first issue treated in this paper.

However, the DNA certification of identity and origin of foodstuffs are not necessarily synonyms of food quality. As an example, the genetic identity of a vineyard influences some aspects of wine quality [32] but other environmental factors could affect the plant phenotype and therefore the wine organoleptic properties [3335]. For these reasons, the DNA-based analysis should be combined with a precise evaluation of chemical food characteristics. The second section of this paper will be devoted to the analysis of modern metabolomics techniques in the field of food science.

Both DNA-based and metabolomics approaches can be simultaneously performed through the so called omics platforms [36], the use of which is expected to progressively become a routine in the context of food control. Given the recent bioinformatics advances, omics platforms are able to process huge amounts of data and combine information belonging to different analytical approaches. Hence, the technological innovations concerning food quality lie in both the development of universal and more accurate analytical systems and their reciprocal integration.

2. DNA Barcoding: A Universal Approach for Food Characterization

As discussed in the previous chapter, an aspect of primary importance in food science is the need to identify the origin of food raw materials, as well as tracing food products along the entire food supply chain by using universal, rapid, and inexpensive tools. In the last decade, “DNA barcoding” was proposed as a universal method to identify living organism including edible plants and animals [37]. The rationale of this approach consists in the analysis of the variability at one or a few standard region/s of the genome (i.e., DNA barcodes) occurring in the whole panel of organisms constituting the raw materials and their derived food products [38].

The 5′-end portion of mitochondrial coxI gene was suggested as standard DNA barcode region for metazoans. In plants, mitochondrial DNA has slower substitution rates and shows intramolecular recombination [39], therefore impeding a reliable species identification. The research for an ideal DNA barcode in terrestrial plants has focused on two plastid DNA regions (i.e., rbcL and matK) considered as the “core-barcode” [40]. These can be supported by other regions, such as the trnH-psbA intergenic spacer, due to their higher variability among congenerics [41, 42]. Internal transcribed spacer regions of nuclear ribosomal DNA (ITS) were also recommended as additional markers in angiosperms [39].

Although there is still much debate on the identification performances of these markers, DNA barcoding showed its effectiveness when used to characterize unknown specimens based on the comparison with reference sequences [42, 43], especially for edible organisms used in food production [4447]. The efficacy of DNA barcoding is supported by the availability of a comprehensive and continuously growing public library of DNA barcodes, the Barcode of Life Data System (BOLD), which provides a global identification system that is freely accessible [48, 49]. This platform consists of several components, including the Identification Engine tool (BOLD-IDS), which works with DNA barcode sequences and returns a taxonomic assignment at the species level whenever possible.

A case in which DNA barcoding works well is the analysis of seafood [50], where coxI showed higher discrimination ability and in several cases allowed the identification of the origin of certain fish stocks. Moreover, in the modern market, many seafood species are sold as fillets or slices, therefore hindering the application of classical identification approaches. In such cases, the molecular analysis is the only reliable strategy to identify species [51]. Given its efficacy, DNA barcoding was adopted by the US Food and Drug Administration for the authentication of fish-based commercial products [52].

A limited success of the method was achieved concerning meat identification, especially concerning farmed species. The main reason of this pitfall lies in the scarce variability of the conventional barcode region among animal breeds and in the frequent occurrence of hybridization events [53]. In contrast, regarding dairy products, DNA barcoding has been proven efficient in characterizing composition and origin of milk. Indeed, the plastidial rbcL barcode marker was found to be able to detect traces of food-derived plant DNA fragments in raw cow milk [54, 55], thus opening new perspectives for the traceability of milk and dairy products in general.

Among plant-based foodstuffs, the DNA barcoding approach has been used for many applications [56] and to investigate the genetic relationships between wild and cultivated plants, as well as their origin. As an example, DNA barcoding was used to characterize the bean germplasm (Phaseolus vulgaris L.) and was found able to distinguish among different haplotypes of bean accessions from the Mesoamerican and Andean areas [57]. Similarly, the DNA barcoding approach was adopted to assess the origin and quality of spices [44, 58], herbal products [59, 60], and naturally processed plant products such as multiflower honey [61]. Other studies investigated the ability of DNA barcoding in discerning toxic plants from edible species: cultivated species of the genera Solanum and Prunus were successfully distinguished from their toxic congenerics [62] and from some frequent plant species misidentifications that cause poisoning in human [63].

On the whole, the most important innovation introduced by DNA barcoding is the merging in a single approach of three characteristics typical of molecular analytic tools: (i) the molecularization of identification processes (i.e., the investigation of DNA variability to discriminate among taxa); (ii) the standardization of molecular marker/s and of analytical procedures; (iii) the data computerization of identification results (i.e., the not redundant transposition of the data using informatics) [64]. This last element is fundamental to make the analytic DNA-based tool accessible to the different actors involved in the food supply chain. Table 1 provides an updated list of DNA barcoding case studies dealing with raw materials and foodstuffs with a clear indication of the beneficiary subjects of the analysis: producer, distributor, and consumer.

Table 1: Updated list of DNA barcoding case studies in the field of food science and principal stakeholders. Producers are interested in valuing their crops or breeds by molecular certification; distributors are mainly interested in the traceability and authentication of traded products; the interest of consumers is to avoid commercial frauds/species substitutions and have an assurance on food provenance.

Although DNA barcoding largely demonstrated its high sensitivity and reliability in the authentication of food products, it should be specified that most food products are composed of a mix of organisms. In these cases, the use of universal primers and standard sequencing approaches, based on the traditional Sanger technology, are inefficient to discriminate among the single components. As a result, the requirement for high-throughput sequencing techniques grew by an unpredicted extent [106]. Several novel approaches evolved to replace the traditional Sanger sequencing method; these modern advances have been referred to as “high-throughput DNA sequencing” (HTS). HTS techniques are able to provide billion sequence data several times faster and cheaper than the conventional Sanger approach. The reduction in cost and time for generating DNA sequence data has resulted in a range of new successful applications, including food traceability and especially food microbiology [16, 107]. As an example, HTS techniques have been used to identify fruit species in yogurts [108] and pollen composition in multiflower honeys [109].

Nowadays, the use of DNA barcoding in the food sector moved from the academic research to a real application. The “molecular labelling” provided by DNA barcoding has benefits for both consumers (who are ensured on the origin, quality, and safety of food items) and producers (who can give an additional value to their products or have an assurance on the quality of starting raw materials). Concerning the analytical feasibility of the method, the DNA barcoding tool is easily accessible due to the availability of public molecular reference databases and a lot of equipped public or private laboratories able to perform the analysis. Newmaster and colleagues, in a publication dated 2009, estimated the cost of a single analysis in a few Euro and very short times of response [110]. Federici and colleagues demonstrated that portions of the standard DNA barcodes could be chosen as SCAR markers to discriminate in less than three hours between edible plant species from poisonous ones [63]. These characteristics make DNA barcoding a diagnostic method suitable for food control analyses by national and international agencies. As previously underlined, to assess the origin of food items, DNA-based analyses should be combined with the characterization of food metabolites to obtain an exhaustive molecular label.

3. Innovative Applications of Metabolomics Tools for an Exhaustive Food Labelling

The analysis of food metabolome represents a new frontier in the evaluation of food quality [111]. The metabolome consists of low molecular weight entities (i.e., <1,000 Da) [112] belonging to a wide range of chemical classes, occurring at different concentrations. In general, these metabolites are the final downstream products of the genome and of its interactions with the environment. For this reason, the analysis of genotype only (e.g., DNA barcoding) is certainly important but not exhaustive to evaluate the overall quality of food items.

In food chemistry, some molecules such as sugars are common and abundant, whereas minor compounds like vitamins occur at smaller amounts or even at trace concentrations (e.g., femtomolar). In addition, the physicochemical properties of some groups of molecules, or the patterns of reciprocal interaction, could pose problems to their fine characterization and quantification. Thus, efficient and sensitive analytical tools are required for a reliable characterization of food metabolome. Whilst in DNA fingerprinting approaches the identification is based on the reading of short nucleotide DNA sequences, a metabolomics fingerprinting analysis aims at establishing the patterns of metabolites belonging to different chemical classes and that are correlated to certain characteristics. Thus, one of the main challenges in food metabolomics is facing the complex networks of molecules (e.g., sugars, amino acids, peptides, organic acids, phenols, terpenes, or steroids) occurring in a particular food item. For these reasons, two approaches (profiling and fingerprinting) can be used to characterize the food metabolome. Profiling is a targeted strategy focused on the analysis of a group of related metabolites, often belonging to the same chemical class. An example of this approach is the discrimination between Arabica and Robusta coffee origins, based on the identification and quantification of a specific class of molecules, including 16-O-Methylcafestol, by NMR spectroscopy [113]. In addition, very recently, Monti and coworkers discriminated among different peach qualities and level of ripening, which depend on the abundance of several metabolites, including amino acids, sugars, and organic acids [114]. The second approach (fingerprinting), is an untargeted strategy based on comparing patterns of metabolites among different samples using chemometric tools. The main aim of fingerprinting is not to identify all the involved compounds but to establish patterns among them; this approach enables the simultaneous detection of a wide class of metabolites. Examples of metabolic fingerprinting on different foodstuffs include grape and wine [115, 116], orange [117], saffron [118], olive oil [119], and wheat and bread [120]. Profiling and fingerprinting can offer complementary information and thus can be used alone or in combination [121, 122].

Independently from the adopted strategy, a reliable tool to analyse the metabolome of a certain food should ideally meet some features: (i) the possibility of recognizing a variety of chemical structures, (ii) the possibility of dealing with large range of concentrations at which metabolites are present in a matrix, (iii) the capability of the analytical platforms, and (iv) the availability of reference databases with extensive details and descriptors [123].

Today, there are two analytical platforms meeting these criteria: Nuclear Magnetic Resonance (NMR) spectroscopy and Mass Spectrometry (MS) [121]. The application of NMR and MS techniques greatly increased in the last years (Figure 1(a)) and this research field covers several subject areas and disciplines (Figure 1(b)).

Figure 1: (a) Studies published in the area of food research, based on NMR and/or MS analyses, from 2001 to 2014. (b) NMR- and/or MS-based studies published from 2001 to 2014, divided for subject area. Source: Scopus (entries: NMR, food or Mass Spectrometry, food).

A good advantage of both techniques is the “high-throughput” capability of spectroscopic and structural information that permits characterizing a wide range of metabolites simultaneously, with high analytical precision. Compared to NMR, MS is more sensitive and can be used alone or combined with gas chromatography, liquid chromatography, or capillary electrophoresis to provide a higher sensitivity for metabolites present at low or even at trace concentrations [124127]. However, even though MS-based analytical methods can detect hundreds of metabolites, many others could remain unidentified. On the other side, the main advantages of NMR are the ease sample preparation and the determination of very different chemical species in a single experiment. In addition, the identification of molecules is easier and more straightforward than in the case of MS. Other important advantages of NMR are its inherently quantitative signals and its nontargeted and nondestructive nature with regard to the specimen of the technique. Thus, in case of an initial metabolomics study where the composition of the metabolite pool is not known, a NMR approach is useful and can inform future studies by targeted GC-MS metabolomics or other approaches to look for specific low-concentration metabolites (targeted strategy). NMR sensitivity is considered one of the main limitations in its application to metabolomics analysis, especially when compared to MS. However, continuous developments in hardware (e.g., magnet strength, probe head design, and console electronics) have allowed and will allow a growing sensitivity of NMR. Also, a rapid growth in new, potent algorithms for multivariate data analysis facilitates the use of NMR spectroscopy as a competitive, complementary analytical platform for investigating the food metabolome (Table 2).

Table 2: Examples of NMR and MS application in the field of food science.

The most important innovation provided by metabolomics tools is their standardization and the universality of the procedures. The amount of data generated by these analyses is enormous. For this reason, several chemometric tools [139, 140] are employed. In fact, to analyze food metabolomics data, some intermediate steps are necessary, including peak detection, spectra normalization, integration, and data alignment before multivariate statistical analysis.

Based on these aspects, it is currently possible to create a molecular label, which combines the genetic profile of a certain food item and its metabolic content. The advantages of such integration are relevant and would certainly constitute a real innovation in food science. One example is the case of wine, which can be putatively characterized with both DNA analysis of the original grape cultivar (e.g., [141, 142]) and the metabolic profile to identify wine characteristics, such as fermentation behaviours and antioxidant properties. Indeed, the analysis of metabolome was shown successful in identifying specific chemical compounds strictly related to the geographic production areas [115, 143]. The origin of wine could also be supported by the DNA-based analysis of must/wine microbiome [144146]. Merging these three sources of data would result in a molecular label that is truly exhaustive and follows the Protected Designation of Origin (PDO) of wine.

Another application of metabolomics was on olive oil. Longobardi et al. [119] used a1H NMR fingerprinting combined with multivariate statistical analysis to authenticate extra virgin olive oils from seven different Mediterranean regions, demonstrating the possibility to predict the origin of olive oil samples with a very high confidence (>78%). At the DNA level, DNA barcoding cannot distinguish among different olive cultivars, whereas other genomics markers such as SSR and SNP were successful in achieving this goal [147]. DNA barcoding, combined with HRM (High Resolution Melting) analysis, was used instead to detect adulteration of olive oil with other oils [148]. Also in this case, genomics and metabolomics analyses could be complementary, to offer to the producer/consumer a comprehensive certification of origin and quality of oil.

An important aspect of food metabolome is that of flavour and aroma determination, which is often linked to the composition in volatile molecules. Dynamic headspace solid-phase microextraction (HS-SPME) followed by GC separation and high resolution MS analyser can be exploited to characterize the volatile components of some foodstuffs. With this approach, the volatile metabolomics pattern of beer raw materials has been defined in a recent paper [134]. Similar results were obtained with aromatic spices [149, 150] that have been also characterized using DNA barcoding approaches [44, 76]. In a strict sense, these results indicate that in the case of spices it is possible not only to identify the species but also the peculiar aromatic components responsible for their flavour and scent. Such combined analytical system can be seen as a way to also evaluate the efficacy of the processing of spices-based products along the entire supply chain (e.g., harvesting, exsiccation, grinding, and packaging).

Taking advantage of all these features and tools, NMR and MS are today able to answer most issues related to food analysis: (i) food traceability, authenticity, and safety, (ii) food composition and physical characteristics, (iii) food processing and storage, and (iv) food and health.

Thus, the study of the whole metabolic profile of food products can help defining quality features that make certain foods unique and can bring information on food safety and authenticity. For example, genetic modification, microorganisms colonization, and other food characteristics of major concern for human health are likely to influence large portions of the raw material or processed food molecular profile.

Another advantage of including the characteristics of the metabolome in the molecular label of a certain food is the potential of metabolomics in evaluating critical steps of the supply chain such as production, storage, and distribution. In 2014, Gallo and colleagues [116] described an interesting NMR application to study the influence of agronomical practices on the chemical composition of commercial table grapes. Specifically, the variability of the grape metabolome composition was evaluated considering primary metabolites, the compounds directly involved in the growth, and development of fruits. The authors found glucose, fructose, arginine, and ethanol as compounds quantitatively influenced by farming practices. Moreover, the comparison between organic and conventional productions showed a higher sugar content for the latter, resulting in a higher sugar-to-acid ratio [116].

In such a context, a metabolomics approach is complementary to a DNA barcoding analysis in evaluating the production processes as well as in monitoring the occurrence of alterations and species substitutions cases. For example, in 2015, Cagliani et al. [118] published an interesting application of metabolomics to characterize saffron, a very expensive and PDO spice. By using a multivariate statistical analysis of NMR data, they identified reliable biomarkers, specifically picrocrocin and crocins that permit distinguishing Italian products from other commercial varieties, where these peculiar compounds are less abundant (or even absent) [118].

The availability of an analytical platform based on the combination of genomics and metabolomics tools will have great potential in terms of food safety. As underlined in the first chapter, since its introduction in the 90’s, the DNA-based diagnostics has developed different strategies to detect food pathogenic organisms. A DNA barcoding approach, combined with the use of HTS technologies, could certainly provide great advantages in this field because it would permit obtaining a comprehensive vision of all the putative food-related pathogens. However, this integrative panel of data would not be completely exhaustive because some microorganisms could be dead or inactive or become pathogenic only when they release specific toxins or metabolites [151, 152]. In this context, a metabolomics analysis based on MS/NMR approaches could provide important information regarding the occurrence of these metabolites or other compounds of major concern (e.g., antibiotics and pesticides) in foodstuffs. A rapid and simple analytical method, able to identify 255 veterinary drug residues in raw milk, was developed by Zhan and coworkers [128]. Their method was based on a two-step precipitation and ultra performance liquid chromatography coupled with electrospray ionization and tandem Mass Spectrometry (UPLC–ESI–MS/MS). Malachová et al. [129] optimized and validated in 2014 a LC–MS/MS method for the detection of 295 fungal and bacterial metabolites in four different types of food matrices: apple puree for infants (high water content), hazelnuts (high fat content), maize (high starch and low fat content), and green pepper (difficult or unique matrix).

Finally, recent studies have shown the possibility to link the metabolic profiling and characterization of foodstuffs to the screening of food matrices, aiming at the identification of small molecules able to bind and modulate the activity of a target protein (often involved in the etiology of specific pathologies). Techniques such as Saturation Transfer Difference- (STD-) NMR [153155] and trNOESY NMR experiments [156, 157] allowed the identification of natural ligands present in Salvia sclareoides [136] and green tea [137], able to recognize, bind, and modulate the activity of Aβ peptides (whose aggregation processes are considered among the main biochemical events leading to Alzheimer’s disease).

In conclusion, the future of food analysis will necessarily be based on the exploitation of integrative approaches, including both genomics and metabolomics. If in the past this was not feasible because of the lack of expertise and technical limitations, the current technological advances offer high performances in terms of standardization and universality to investigate a wide panel of food items. The spread of omics platforms, able to simultaneously process different matrices with a multiapproach strategy [111], unified under the control of bioinformatics tools, is boosting this revolution.

4. From Omics to Foodomics

The use of omics platforms to assess important aspects of food items (i.e., contaminants and bioactive molecules) is essential to obtain an exhaustive characterization of food quality and safety or to assess the effect of food on human cells, tissues, and organs as well. The availability of such platforms responds to a general trend in food science about the linking between food and health [7]. Nowadays, food is more and more considered not only as a source of energy but also as an affordable way to prevent future diseases. In this scenario, human health should be considered as a dynamic position in a multidimensional space [158] that spans from growth to development to reproduction. Early nutritional events (i.e., since the embryonic state) and food imprinting can define the trajectories of development and contribute to the wellness or the insurgence of noncommunicable diseases such as allergy, diabetes, and obesity [159]. In the development and maintaining ages, a proper nutrition could offer the better cost effective way to prevent such noncommunicable diseases [160]. Furthermore, undernutrition and overweight are global problems. The “global nutrition report” of 2013 highlights how the world is off-track to meet the 2025 World Health Assembly targets for nutrition [161]. Apart from social and economical issues, from the scientific point of view, nutrition research can furnish the keys for defining the characteristics of a proper nutrition. Therefore, a new discipline known as “foodomics” has been defined to study the food and nutrition domains through the application of advanced omics technologies to improve consumer’s well-being, health, and confidence [162, 163]. Thanks to foodomics, many issues related to food could be addressed such as the evaluation of the effects of certain bioactive food components on biochemical, molecular, and cellular mechanisms, or the identification of gene-based differences among individuals in response to a specific dietary pattern [164166]. Foodomics tools could permit identifying molecular biomarkers strictly related to the genes involved in the early stages of a certain disease and to elucidate the effect of bioactive food constituents on crucial molecular pathways for preventing future diseases with an adequate diet [166168]. For example, a foodomics analysis was used to evaluate the effect of dietary polyphenols against colon cancer [169]. Ibáñez and coworkers [169] tested the chemopreventive effect of polyphenols from rosemary on the total gene, protein, and metabolite expression in human HT29 colon cancer cells. The results obtained from each component of the omics platform (i.e., transcriptomics, proteomics, and metabolomics) were integrated to estimate which cellular pathways were activated in response to polyphenols. Data suggests that polyphenols bring about an induction of cell-cycle arrest, an increase of apoptosis, and an improvement of cellular antioxidant activity. The genes, proteins, and metabolites involved in these three processes were identified thanks to the multiparameter omics analysis. It is important to underline the fact that the induction of apoptosis is especially relevant in colon cancer, since the renewal of the colon epithelium via apoptosis is the way used by the organism to eliminate deteriorated cells that can mutate to carcinogenic. Therefore, a diet rich in polyphenols plays an important role in the prevention of colon cancer.

Foodomics is a powerful discipline to identify the adding value properties of food items, as well as to detect food-related toxins and allergens or to assess the effects of food on human metabolism by evaluating cell-response [170, 171]. The efficacy of omics in the food sector also meets the emerging needs related to personalized nutrition [172]. A number of recent studies underlined the enormous variability of individual response to the same diet or food components: it is well known that food ingredients have effects that are unique to each individual, as unique as is its own transcriptome, proteome, and metabolome [158]. The role of foodomics does not finish once a personalized diet has been identified. Indeed, an exhaustive evaluation of the factors altering the metabolic properties of food components should also be taken into account. These factors include production process, methods, and duration of conservation, interaction with other components, cooking procedures, digestion, and interaction with microbiome [173].

The advantages of foodomics are relevant not only for producers but also for consumers to encourage a healthy diet and to reduce educational, behavioural, and economic barriers to accessing wellness. In this context, recent smartphone “apps” are becoming a powerful tool to promote the consumption of high-quality foodstuffs and in particular the consumption of those food items able to prevent diseases [174177]. Such informative tools (including online portals and dissemination web sites) can be useful for different stakeholders to translate a molecular label based on omics approaches in a more understandable language for the whole category of consumers. The molecular labelling that combines DNA barcoding and metabolomics data with the information of foodomics represents a precious source of data to meet consumer requirements. In this sense, smartphone apps represent a simple tool able to share and translate molecular data to the various stakeholders of the food supply chain.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

References

  1. A. Galimberti, F. De Mattia, A. Losa et al., “DNA barcoding as a new tool for food traceability,” Food Research International, vol. 50, no. 1, pp. 55–63, 2013. View at Publisher · View at Google Scholar · View at Scopus
  2. C. Byrd-Bredbenner, M. N. Cohn, J. M. Farber et al., “Food safety considerations for innovative nutrition solutions,” Annals of the New York Academy of Sciences, vol. 1347, no. 1, pp. 29–44, 2015. View at Publisher · View at Google Scholar
  3. B. C. Remington, J. L. Baumert, W. M. Blom, G. F. Houben, S. L. Taylor, and A. G. Kruizinga, “Unintended allergens in precautionary labelled and unlabelled products pose significant risks to UK allergic consumers,” Allergy, vol. 70, no. 7, pp. 813–819, 2015. View at Publisher · View at Google Scholar
  4. W. Van Rijswijk and L. J. Frewer, “Consumer perceptions of food quality and safety and their relation to traceability,” British Food Journal, vol. 110, no. 10, pp. 1034–1046, 2008. View at Publisher · View at Google Scholar · View at Scopus
  5. W. Van Rijswijk and L. J. Frewer, “Consumer needs and requirements for food and ingredient traceability information,” International Journal of Consumer Studies, vol. 36, no. 3, pp. 282–290, 2012. View at Publisher · View at Google Scholar · View at Scopus
  6. G. Nocella, D. Romano, and G. Stefani, “Consumers' attitudes, trust and willingness to pay for food information,” International Journal of Consumer Studies, vol. 38, no. 2, pp. 153–165, 2014. View at Publisher · View at Google Scholar · View at Scopus
  7. V. García-Cañas, C. Simó, M. Herrero, E. Ibáñez, and A. Cifuentes, “Present and future challenges in food analysis: foodomics,” Analytical Chemistry, vol. 84, no. 23, pp. 10150–10159, 2012. View at Publisher · View at Google Scholar · View at Scopus
  8. M. J. Myers, “Molecular identification of animal species in food: transition from research laboratories to the regulatory laboratories,” Veterinary Journal, vol. 190, no. 1, pp. 7–8, 2011. View at Publisher · View at Google Scholar · View at Scopus
  9. V. Fusco and G. M. Quero, “Culture-dependent and culture-independent nucleic-acid-based methods used in the microbial safety assessment of milk and dairy products,” Comprehensive Reviews in Food Science and Food Safety, vol. 13, no. 4, pp. 493–537, 2014. View at Publisher · View at Google Scholar · View at Scopus
  10. D. G. Newell, M. Koopmans, L. Verhoef et al., “Food-borne diseases—the challenges of 20 years ago still persist while new ones continue to emerge,” International Journal of Food Microbiology, vol. 139, no. 1, pp. S3–S15, 2010. View at Publisher · View at Google Scholar · View at Scopus
  11. B. R. Jackson, P. M. Griffin, D. Cole, K. A. Walsh, and S. J. Chai, “Outbreak-associated Salmonella enterica serotypes and food commodities, United States, 1998–2008,” Emerging Infectious Diseases, vol. 19, no. 8, pp. 1239–1244, 2013. View at Publisher · View at Google Scholar · View at Scopus
  12. L. Settanni and A. Corsetti, “The use of multiplex PCR to detect and differentiate food- and beverage-associated microorganisms: a review,” Journal of Microbiological Methods, vol. 69, no. 1, pp. 1–22, 2007. View at Publisher · View at Google Scholar · View at Scopus
  13. M. P. Doyle and R. L. Buchanan, Eds., Food Microbiology: Fundamentals and Frontiers, ASM Press, Washington, DC, USA, 4th edition, 2013.
  14. D. Ercolini, “High-throughput sequencing and metagenomics: moving forward in the culture-independent analysis of food microbial ecology,” Applied and Environmental Microbiology, vol. 79, no. 10, pp. 3148–3155, 2013. View at Publisher · View at Google Scholar · View at Scopus
  15. S. Ceuppens, D. Li, M. Uyttendaele et al., “Molecular methods in food safety microbiology: interpretation and implications of nucleic acid detection,” Comprehensive Reviews in Food Science and Food Safety, vol. 13, no. 4, pp. 551–577, 2014. View at Publisher · View at Google Scholar · View at Scopus
  16. A. Galimberti, A. Bruno, V. Mezzasalma, F. De Mattia, I. Bruni, and M. Labra, “Emerging DNA-based technologies to characterize food ecosystems,” Food Research International, vol. 69, pp. 424–433, 2015. View at Publisher · View at Google Scholar · View at Scopus
  17. A. M. Almeida, A. Bassols, E. Bendixen et al., “Animal board invited review: advances in proteomics for animal and food sciences,” Animal, vol. 9, no. 1, pp. 1–17, 2014. View at Publisher · View at Google Scholar · View at Scopus
  18. I. Mafra, I. M. P. L. V. O. Ferreira, and M. B. P. P. Oliveira, “Food authentication by PCR-based methods,” European Food Research and Technology, vol. 227, no. 3, pp. 649–665, 2008. View at Publisher · View at Google Scholar · View at Scopus
  19. Y.-J. Park, J. K. Lee, and N.-S. Kim, “Simple sequence repeat polymorphisms (SSRPs) for evaluation of molecular diversity and germplasm classification of minor crops,” Molecules, vol. 14, no. 11, pp. 4546–4569, 2009. View at Publisher · View at Google Scholar · View at Scopus
  20. T. Bauer, P. Weller, W. P. Hammes, and C. Hertel, “The effect of processing parameters on DNA degradation in food,” European Food Research and Technology, vol. 217, no. 4, pp. 338–343, 2003. View at Publisher · View at Google Scholar · View at Scopus
  21. Ö. Aslan, R. M. Hamill, T. Sweeney, W. Reardon, and A. M. Mullen, “Integrity of nuclear genomic deoxyribonucleic acid in cooked meat: implications for food traceability,” Journal of Animal Science, vol. 87, no. 1, pp. 57–61, 2009. View at Publisher · View at Google Scholar · View at Scopus
  22. M. T. Cimino, “Successful isolation and PCR amplification of DNA from national institute of standards and technology herbal dietary supplement standard reference material powders and extracts,” Planta Medica, vol. 76, no. 5, pp. 495–497, 2010. View at Publisher · View at Google Scholar · View at Scopus
  23. J. P. Llongueras, S. Nair, D. Salas-Leiva, and A. E. Schwarzbach, “Comparing DNA extraction methods for analysis of botanical materials found in anti-diabetic supplements,” Molecular Biotechnology, vol. 53, no. 3, pp. 249–256, 2013. View at Publisher · View at Google Scholar · View at Scopus
  24. D. I. Ellis, V. L. Brewster, W. B. Dunn, J. W. Allwood, A. P. Golovanov, and R. Goodacre, “Fingerprinting food: current technologies for the detection of food adulteration and contamination,” Chemical Society Reviews, vol. 41, no. 17, pp. 5706–5727, 2012. View at Publisher · View at Google Scholar · View at Scopus
  25. P. Kumar, V. K. Gupta, A. K. Misra, D. R. Modi, and B. K. Pandey, “Potential of molecular markers in plant biotechnology,” Plant Omics Journal, vol. 2, no. 4, pp. 141–162, 2009. View at Google Scholar
  26. M. Labra, S. Imazio, F. Grassi et al., “Molecular approach to assess the origin of cv. Marzemino,” Vitis, vol. 42, no. 3, pp. 137–140, 2003. View at Google Scholar · View at Scopus
  27. F. De Mattia, S. Imazio, F. Grassi et al., “Genetic characterization of Sardinia grapevine cultivars by SSR markers analysis,” Journal International des Sciences de la Vigne et du Vin, vol. 41, no. 4, pp. 175–184, 2007. View at Google Scholar · View at Scopus
  28. I. J. Nijman, M. Otsen, E. L. C. Verkaar et al., “Hybridization of banteng (Bos javanicus) and zebu (Bos indicus) revealed by mitochondrial DNA, satellite DNA, AFLP and microsatellites,” Heredity, vol. 90, no. 1, pp. 10–16, 2003. View at Publisher · View at Google Scholar · View at Scopus
  29. The Bovine HapMap Consortium, “Genome-wide survey of SNP variation uncovers the genetic structure of cattle breeds,” Science, vol. 324, no. 5926, pp. 528–532, 2009. View at Publisher · View at Google Scholar
  30. N. Z. Ballin, “Authentication of meat and meat products,” Meat Science, vol. 86, no. 3, pp. 577–587, 2010. View at Publisher · View at Google Scholar · View at Scopus
  31. H.-Y. Chuang, H.-S. Lur, K.-K. Hwu, and M.-C. Chang, “Authentication of domestic Taiwan rice varieties based on fingerprinting analysis of microsatellite DNA markers,” Botanical Studies, vol. 52, no. 4, pp. 393–405, 2011. View at Google Scholar · View at Scopus
  32. M. Labra, M. Winfield, A. Ghiani et al., “Genetic studies on Trebbiano and morphologically related varieties by SSR and AFLP markers,” Vitis, vol. 40, no. 4, pp. 187–190, 2001. View at Google Scholar · View at Scopus
  33. G. V. Jones, M. A. White, O. R. Cooper, and K. Storchmann, “Climate change and global wine quality,” Climatic Change, vol. 73, no. 3, pp. 319–343, 2005. View at Publisher · View at Google Scholar · View at Scopus
  34. G. Martins, C. Miot-Sertier, B. Lauga et al., “Grape berry bacterial microbiota: impact of the ripening process and the farming system,” International Journal of Food Microbiology, vol. 158, no. 2, pp. 93–100, 2012. View at Publisher · View at Google Scholar · View at Scopus
  35. R. Ferrise, G. Trombi, M. Moriondo, and M. Bindi, “Climate change and grapevines: a simulation study for the mediterranean basin,” Journal of Wine Economics, pp. 1–17, 2014. View at Publisher · View at Google Scholar
  36. M. L. Jiménez-Pranteda, A. Pérez-Davó, M. Monteoliva-Sánchez, A. Ramos-Cormenzana, and M. Aguilera, “Food omics validation: towards understanding key features for gut microbiota, probiotics and human health,” Food Analytical Methods, vol. 8, no. 2, pp. 272–289, 2015. View at Publisher · View at Google Scholar · View at Scopus
  37. M. Casiraghi, M. Labra, E. Ferri, A. Galimberti, and F. de Mattia, “DNA barcoding: a six-question tour to improve users' awareness about the method,” Briefings in Bioinformatics, vol. 11, no. 4, pp. 440–453, 2010. View at Publisher · View at Google Scholar · View at Scopus
  38. P. D. N. Hebert, S. Ratnasingham, and J. R. de Waard, “Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species,” Proceedings of the Royal Society B: Biological Sciences, vol. 270, supplement 1, pp. S96–S99, 2003. View at Publisher · View at Google Scholar · View at Scopus
  39. P. M. Hollingsworth, S. W. Graham, and D. P. Little, “Choosing and using a plant DNA barcode,” PLoS ONE, vol. 6, no. 5, Article ID e19254, 2011. View at Publisher · View at Google Scholar · View at Scopus
  40. M. L. Hollingsworth, A. Andra Clark, L. L. Forrest et al., “Selecting barcoding loci for plants: evaluation of seven candidate loci with species-level sampling in three divergent groups of land plants,” Molecular Ecology Resources, vol. 9, no. 2, pp. 439–457, 2009. View at Publisher · View at Google Scholar · View at Scopus
  41. W. J. Kress, D. L. Erickson, N. G. Swenson, J. Thompson, M. Uriarte, and J. K. Zimmerman, “Advances in the use of DNA barcodes to build a community phylogeny for tropical trees in a Puerto Rican forest dynamics plot,” PLoS ONE, vol. 5, no. 11, Article ID e15409, 2010. View at Publisher · View at Google Scholar
  42. A. Galimberti, F. De Mattia, I. Bruni et al., “A DNA barcoding approach to characterize pollen collected by honeybees,” PLoS ONE, vol. 9, no. 10, Article ID e108344, 2014. View at Publisher · View at Google Scholar · View at Scopus
  43. K. S. Burgess, A. J. Fazekas, P. R. Kesanakurti et al., “Discriminating plant species in a local temperate flora using the rbcL+matK DNA barcode,” Methods in Ecology and Evolution, vol. 2, no. 4, pp. 333–340, 2011. View at Publisher · View at Google Scholar · View at Scopus
  44. F. De Mattia, I. Bruni, A. Galimberti, F. Cattaneo, M. Casiraghi, and M. Labra, “A comparative study of different DNA barcoding markers for the identification of some members of Lamiacaea,” Food Research International, vol. 44, no. 3, pp. 693–702, 2011. View at Publisher · View at Google Scholar · View at Scopus
  45. P. Gaubert, F. Njiokou, A. Olayemi et al., “Bushmeat genetics: setting up a reference framework for the DNA typing of African forest bushmeat,” Molecular Ecology Resources, vol. 15, no. 3, pp. 633–651, 2015. View at Publisher · View at Google Scholar
  46. H. A. Raja, T. R. Baker, J. G. Little, and N. H. Oberlies, “DNA barcoding for identification of species in mushrooms: a component of product certification,” Planta Medica, vol. 80, no. 10, article PR4, 2014. View at Publisher · View at Google Scholar
  47. R. Khaksar, T. Carlson, D. W. Schaffner et al., “Unmasking seafood mislabeling in U.S. markets: DNA barcoding as a unique technology for food authentication and quality control,” Food Control, vol. 56, pp. 71–76, 2015. View at Publisher · View at Google Scholar
  48. S. Ratnasingham and P. D. N. Hebert, “BOLD: the barcode of life data system (http://www.barcodinglife.org),” Molecular Ecology Notes, vol. 7, no. 3, pp. 355–364, 2007. View at Publisher · View at Google Scholar
  49. S. Ratnasingham and P. D. N. Hebert, “A DNA-based registry for all animal species: the Barcode Index Number (BIN) System,” PLoS ONE, vol. 8, no. 7, Article ID e66213, 2013. View at Publisher · View at Google Scholar · View at Scopus
  50. S. Becker, R. Hanner, and D. Steinke, “Five years of FISH-BOL: brief status report,” Mitochondrial DNA, vol. 22, no. 1, pp. 3–9, 2011. View at Publisher · View at Google Scholar · View at Scopus
  51. M. Barbuto, A. Galimberti, E. Ferri et al., “DNA barcoding reveals fraudulent substitutions in shark seafood products: the Italian case of ‘palombo’ (Mustelus spp.),” Food Research International, vol. 43, no. 1, pp. 376–381, 2010. View at Publisher · View at Google Scholar · View at Scopus
  52. H. F. Yancy, T. S. Zemlak, J. A. Mason et al., “Potential use of DNA barcodes in regulatory science: applications of the regulatory fish encyclopedia,” Journal of Food Protection, vol. 71, no. 1, pp. 210–217, 2008. View at Google Scholar · View at Scopus
  53. Y. Kikkawa, T. Takada, Sutopo et al., “Phylogenies using mtDNA and SRY provide evidence for male-mediated introgression in Asian domestic cattle,” Animal Genetics, vol. 34, no. 2, pp. 96–101, 2003. View at Publisher · View at Google Scholar · View at Scopus
  54. A. Nemeth, A. Wurz, L. Artim et al., “Sensitive PCR analysis of animal tissue samples for fragments of endogenous and transgenic plant DNA,” Journal of Agricultural and Food Chemistry, vol. 52, no. 20, pp. 6129–6135, 2004. View at Publisher · View at Google Scholar · View at Scopus
  55. E. Ponzoni, F. Mastromauro, S. Gianì, and D. Breviario, “Traceability of plant diet contents in raw cow milk samples,” Nutrients, vol. 1, no. 2, pp. 251–262, 2009. View at Publisher · View at Google Scholar · View at Scopus
  56. A. Galimberti, M. Labra, A. Sandionigi, A. Bruno, V. Mezzasalma, and F. De Mattia, “DNA barcoding for minor crops and food traceability,” Advances in Agriculture, vol. 2014, Article ID 831875, 8 pages, 2014. View at Publisher · View at Google Scholar
  57. S. Nicolè, D. L. Erickson, D. Ambrosi et al., “Biodiversity studies in Phaseolus species by DNA barcoding,” Genome, vol. 54, no. 7, pp. 529–545, 2011. View at Publisher · View at Google Scholar · View at Scopus
  58. V. A. Parvathy, V. P. Swetha, T. E. Sheeja, and B. Sasikumar, “Detection of plant-based adulterants in turmeric powder using DNA barcoding,” Pharmaceutical Biology, vol. 53, no. 12, pp. 1774–1779, 2015. View at Publisher · View at Google Scholar
  59. A. T. Uncu, A. O. Uncu, A. Frary, and S. Doganlar, “Authentication of botanical origin in herbal teas by plastid non-coding DNA length polymorphisms,” Journal of Agricultural and Food Chemistry, vol. 63, no. 25, pp. 5920–5929, 2015. View at Publisher · View at Google Scholar
  60. P. Mishra, A. Kumar, A. Nagireddy et al., “DNA barcoding: an efficient tool to overcome authentication challenges in the herbal market,” Plant Biotechnology Journal, 2015. View at Publisher · View at Google Scholar
  61. I. Bruni, A. Galimberti, L. Caridi et al., “A DNA barcoding approach to identify plant species in multiflower honey,” Food Chemistry, vol. 170, pp. 308–315, 2015. View at Publisher · View at Google Scholar · View at Scopus
  62. I. Bruni, F. De Mattia, A. Galimberti et al., “Identification of poisonous plants by DNA barcoding approach,” International Journal of Legal Medicine, vol. 124, no. 6, pp. 595–603, 2010. View at Publisher · View at Google Scholar · View at Scopus
  63. S. Federici, D. Fontana, A. Galimberti et al., “A rapid diagnostic approach to identify poisonous plants using DNA barcoding data,” Plant Biosystems, vol. 149, no. 3, 2015. View at Publisher · View at Google Scholar · View at Scopus
  64. M. Casiraghi, M. Labra, E. Ferri, A. Galimberti, and F. De Mattia, “DNA barcoding: theoretical aspects and practical applications,” in Tools for Identifying Biodiversity: Progress and Problems, P. L. Nimis and R. Vignes Lebbe, Eds., pp. 269–273, Edizioni Università di Trieste, Trieste, Italy, 2010. View at Google Scholar
  65. T. Hidayat, A. Pancoro, and D. Kusumawaty, “Utility of matK gene to assess evolutionary relationship of genus Mangifera (anacardiaceae) in Indonesia and Thailand,” Biotropia, vol. 18, no. 2, pp. 74–80, 2011. View at Google Scholar
  66. T. Xin, H. Yao, H. Gao et al., “Super food Lycium barbarum (Solanaceae) traceability via an internal transcribed spacer 2 barcode,” Food Research International, vol. 54, no. 2, pp. 1699–1704, 2013. View at Publisher · View at Google Scholar · View at Scopus
  67. L. Jaakola, M. Suokas, and H. Häggman, “Novel approaches based on DNA barcoding and high-resolution melting of amplicons for authenticity analyses of berry species,” Food Chemistry, vol. 123, no. 2, pp. 494–500, 2010. View at Publisher · View at Google Scholar · View at Scopus
  68. T. Hidayat, F. I. Abdullah, C. Kuppusamy, A. A. Samad, and A. Wagiran, “Molecular identification of Malaysian pineapple cultivar based on internal transcribed spacer region,” APCBEE Procedia, vol. 4, pp. 146–151, 2012. View at Publisher · View at Google Scholar
  69. N. Kane, S. Sveinsson, H. Dempewolf et al., “Ultra-barcoding in cacao (Theobroma spp.; malvaceae) using whole chloroplast genomes and nuclear ribosomal DNA,” American Journal of Botany, vol. 99, no. 2, pp. 320–329, 2012. View at Publisher · View at Google Scholar · View at Scopus
  70. M. R. Enan and A. Ahmed, “DNA barcoding based on plastid matK and RNA polymerase for assessing the genetic identity of date (Phoenix dactylifera L.) cultivars,” Genetics and Molecular Research, vol. 13, no. 2, pp. 3527–3536, 2014. View at Publisher · View at Google Scholar · View at Scopus
  71. R. L. Jarret, “DNA barcoding in a crop genebank: the Capsicum annuum species complex,” The Open Biology Journal, vol. 1, no. 1, pp. 35–42, 2008. View at Publisher · View at Google Scholar
  72. I. Ganopoulos, P. Madesis, N. Darzentas, A. Argiriou, and A. Tsaftaris, “Barcode High Resolution Melting (Bar-HRM) analysis for detection and quantification of PDO ‘fava Santorinis’ (Lathyrus clymenum) adulterants,” Food Chemistry, vol. 133, no. 2, pp. 505–512, 2012. View at Publisher · View at Google Scholar · View at Scopus
  73. P. Madesis, I. Ganopoulos, A. Anagnostis, and A. Tsaftaris, “The application of Bar-HRM (Barcode DNA-High Resolution Melting) analysis for authenticity testing and quantitative detection of bean crops (Leguminosae) without prior DNA purification,” Food Control, vol. 25, no. 2, pp. 576–582, 2012. View at Publisher · View at Google Scholar · View at Scopus
  74. S. Federici, A. Galimberti, F. Bartolucci et al., “DNA barcoding to analyse taxonomically complex groups in plants: the case of Thymus (Lamiaceae),” Botanical Journal of the Linnean Society, vol. 171, no. 4, pp. 687–699, 2013. View at Publisher · View at Google Scholar · View at Scopus
  75. A. Gismondi, F. Fanali, J. M. M. Labarga, M. G. Caiola, and A. Canini, “Crocus sativus L. genomics and different DNA barcode applications,” Plant Systematics and Evolution, vol. 299, no. 10, pp. 1859–1863, 2013. View at Publisher · View at Google Scholar · View at Scopus
  76. V. A. Parvathy, V. P. Swetha, T. E. Sheeja, N. K. Leela, B. Chempakam, and B. Sasikumar, “DNA barcoding to detect chilli adulteration in traded black pepper powder,” Food Biotechnology, vol. 28, no. 1, pp. 25–40, 2014. View at Publisher · View at Google Scholar · View at Scopus
  77. M. Wang, H.-X. Zhao, L. Wang et al., “Potential use of DNA barcoding for the identification of Salvia based on cpDNA and nrDNA sequences,” Gene, vol. 528, no. 2, pp. 206–215, 2013. View at Publisher · View at Google Scholar · View at Scopus
  78. M. Y. Stoeckle, C. C. Gamble, R. Kirpekar, G. Young, S. Ahmed, and D. P. Little, “Commercial teas highlight plant DNA barcode identification successes and obstacles,” Scientific Reports, vol. 1, article 42, 2011. View at Publisher · View at Google Scholar · View at Scopus
  79. V. A. Parvathy, V. p. Swetha, T. E. Sheeja, and B. Sasikumar, “Detection of plant-based adulterants in turmeric powder using DNA barcoding,” Pharmaceutical Biology, vol. 53, no. 12, pp. 1774–1779, 2015. View at Publisher · View at Google Scholar
  80. M. Li, K.-L. Wong, W.-H. Chan et al., “Establishment of DNA barcodes for the identification of the botanical sources of the Chinese ‘cooling’ beverage,” Food Control, vol. 25, no. 2, pp. 758–766, 2012. View at Publisher · View at Google Scholar · View at Scopus
  81. M. Arleo, F. Ruibal, J. Pereyra, E. Miquel, M. Fernández, and C. Martínez, “A DNA-based approach to discriminate between quince and apple in quince jams,” International Food Research Journal, vol. 19, no. 4, pp. 1471–1477, 2012. View at Google Scholar · View at Scopus
  82. B. T. M. Dentinger, M. Y. Didukh, and J.-M. Moncalvo, “Comparing COI and ITS as DNA barcode markers for mushrooms and allies (Agaricomycotina),” PLoS ONE, vol. 6, no. 9, Article ID e25081, 2011. View at Publisher · View at Google Scholar · View at Scopus
  83. P. Khaund and S. R. Joshi, “DNA barcoding of wild edible mushrooms consumed by the ethnic tribes of India,” Gene, vol. 550, no. 1, pp. 123–130, 2014. View at Publisher · View at Google Scholar · View at Scopus
  84. A. Ardura, A. R. Linde, J. C. Moreira, and E. Garcia-Vazquez, “DNA barcoding for conservation and management of Amazonian commercial fish,” Biological Conservation, vol. 143, no. 6, pp. 1438–1443, 2010. View at Publisher · View at Google Scholar · View at Scopus
  85. A. Ardura, S. Planes, and E. Garcia-Vazquez, “Applications of DNA barcoding to fish landings: authentication and diversity assessment,” ZooKeys, vol. 365, pp. 49–65, 2013. View at Publisher · View at Google Scholar · View at Scopus
  86. D. C. Carvalho, R. M. Palhares, M. G. Drummond, and T. B. Frigo, “DNA Barcoding identification of commercialized seafood in South Brazil: a governmental regulatory forensic program,” Food Control, vol. 50, pp. 784–788, 2015. View at Publisher · View at Google Scholar · View at Scopus
  87. P. J. Smith, S. M. McVeagh, and D. Steinke, “DNA barcoding for the identification of smoked fish products,” Journal of Fish Biology, vol. 72, no. 2, pp. 464–471, 2008. View at Publisher · View at Google Scholar · View at Scopus
  88. A. Abdullah and H. Rehbein, “Authentication of raw and processed tuna from Indonesian markets using DNA barcoding, nuclear gene and character-based approach,” European Food Research and Technology, vol. 239, no. 4, pp. 695–706, 2014. View at Publisher · View at Google Scholar · View at Scopus
  89. A. Galal-Khallaf, A. Ardura, K. Mohammed-Geba, Y. J. Borrell, and E. Garcia-Vazquez, “DNA barcoding reveals a high level of mislabeling in Egyptian fish fillets,” Food Control, vol. 46, pp. 441–445, 2014. View at Publisher · View at Google Scholar · View at Scopus
  90. A. Mottola, P. Marchetti, M. Bottaro, and A. Di Pinto, “DNA barcoding for species identification in prepared fishery products,” Albanian Journal of Agricultural Sciences, vol. 13, pp. 447–453, 2014. View at Google Scholar
  91. M. A. de Brito, H. Schneider, I. Sampaio, and S. Santos, “DNA barcoding reveals high substitution rate and mislabeling in croaker fillets (Sciaenidae) marketed in Brazil: the case of ‘pescada branca’ (Cynoscion leiarchus and Plagioscion squamosissimus),” Food Research International, vol. 70, pp. 40–46, 2015. View at Publisher · View at Google Scholar · View at Scopus
  92. A. Di Pinto, P. Marchetti, A. Mottola et al., “Species identification in fish fillet products using DNA barcoding,” Fisheries Research, vol. 170, pp. 9–13, 2015. View at Publisher · View at Google Scholar
  93. B. A. Maralit, R. D. Aguila, M. F. H. Ventolero, S. K. L. Perez, D. A. Willette, and M. D. Santos, “Detection of mislabeled commercial fishery by-products in the Philippines using DNA barcodes and its implications to food traceability and safety,” Food Control, vol. 33, no. 1, pp. 119–125, 2013. View at Publisher · View at Google Scholar · View at Scopus
  94. K. Nagalakshmi, P. Annam, G. Venkateshwarlu, G. Pathakota, and W. S. Lakra, “Mislabeling in Indian seafood: an investigation using DNA barcoding,” Food Control, vol. 59, pp. 196–200, 2016. View at Publisher · View at Google Scholar
  95. R. Lamendin, K. Miller, and R. D. Ward, “Labelling accuracy in Tasmanian seafood: an investigation using DNA barcoding,” Food Control, vol. 47, pp. 436–443, 2015. View at Publisher · View at Google Scholar · View at Scopus
  96. A. M. Pappalardo and V. Ferrito, “DNA barcoding species identification unveils mislabeling of processed flatfish products in southern Italy markets,” Fisheries Research, vol. 164, pp. 153–158, 2015. View at Publisher · View at Google Scholar · View at Scopus
  97. A. Armani, L. Guardone, R. La Castellana, D. Gianfaldoni, A. Guidi, and L. Castigliego, “DNA barcoding reveals commercial and health issues in ethnic seafood sold on the Italian market,” Food Control, vol. 55, pp. 206–214, 2015. View at Publisher · View at Google Scholar
  98. P. A. Haye, N. I. Segovia, R. Vera, M. D. L. Á. Gallardo, and C. Gallardo-Escárate, “Authentication of commercialized crab-meat in Chile using DNA Barcoding,” Food Control, vol. 25, no. 1, pp. 239–244, 2012. View at Publisher · View at Google Scholar · View at Scopus
  99. V. R. Vartak, R. Narasimmalu, P. K. Annam, D. P. Singh, and W. S. Lakra, “DNA barcoding detected improper labelling and supersession of crab food served by restaurants in India,” Journal of the Science of Food and Agriculture, vol. 95, no. 2, pp. 359–366, 2015. View at Publisher · View at Google Scholar · View at Scopus
  100. H. Kim, K. S. Kumar, S. Y. Hwang, B. Kang, H. Moon, and K. Shin, “Utility of stable isotope and COI gene sequencing analysis in inferring origin and authentication of Hairtail fish and Shrimp,” Journal of Agricultural and Food Chemistry, vol. 63, no. 22, pp. 5548–5556, 2015. View at Publisher · View at Google Scholar
  101. F. Debenedetti, A. Dalmasso, M. T. Bottero et al., “Application of DNA barcoding for controlling of the species from Octopus genus,” Italian Journal of Food Safety, vol. 3, no. 4, pp. 196–199, 2014. View at Publisher · View at Google Scholar · View at Scopus
  102. S. Bitanyi, G. Bjørnstad, M. Nesje, E. M. Ernest, R. H. Mdegela, and K. H. Røed, “Molecular identification versus local people's information for accurate estimates of bushmeat utilization from the Serengeti ecosystem, Tanzania,” African Journal of Biotechnology, vol. 11, no. 1, pp. 243–252, 2013. View at Publisher · View at Google Scholar · View at Scopus
  103. C. A. Quinto, R. Tinoco, and R. S. Hellberg, “DNA barcoding reveals mislabeling of game meat species on the US commercial market,” Food Control, vol. 59, pp. 386–392, 2016. View at Publisher · View at Google Scholar
  104. D. E. Kane and R. S. Hellberg, “Identification of species in ground meat products sold on the US commercial market using DNA-based methods,” Food Control, vol. 59, pp. 158–163, 2015. View at Publisher · View at Google Scholar
  105. Y. Cai, L. Zhang, F. Shen et al., “DNA barcoding of 18 species of Bovidae,” Chinese Science Bulletin, vol. 56, no. 2, pp. 164–168, 2011. View at Publisher · View at Google Scholar · View at Scopus
  106. L. Solieri, T. C. Dakal, and P. Giudici, “Next-generation sequencing and its potential impact on food microbial genomics,” Annals of Microbiology, vol. 63, no. 1, pp. 21–37, 2013. View at Publisher · View at Google Scholar · View at Scopus
  107. P. Madesis, I. Ganopoulos, I. Sakaridis, A. Argiriou, and A. Tsaftaris, “Advances of DNA-based methods for tracing the botanical origin of food products,” Food Research International, vol. 60, pp. 163–172, 2014. View at Publisher · View at Google Scholar · View at Scopus
  108. A. Ortola-Vidal, H. Schnerr, M. Rojmyr, F. Lysholm, and A. Knight, “Quantitative identification of plant genera in food products using PCR and Pyrosequencing technology,” Food Control, vol. 18, no. 8, pp. 921–927, 2007. View at Publisher · View at Google Scholar · View at Scopus
  109. A. Valentini, C. Miquel, and P. Taberlet, “DNA barcoding for honey biodiversity,” Diversity, vol. 2, no. 4, pp. 610–617, 2010. View at Publisher · View at Google Scholar · View at Scopus
  110. S. G. Newmaster, S. Ragupathy, and J. Janovec, “A botanical renaissance: state-of-the-art DNA bar coding facilitates an automated identification technology system for plants,” International Journal of Computer Applications in Technology, vol. 35, no. 1, pp. 50–60, 2009. View at Publisher · View at Google Scholar · View at Scopus
  111. F. R. Pinu, “Metabolomics—the new frontier in food safety and quality research,” Food Research International, vol. 72, pp. 80–81, 2015. View at Publisher · View at Google Scholar
  112. E. Trujillo, C. Davis, and J. Milner, “Nutrigenomics, proteomics, metabolomics, and the practice of dietetics,” Journal of the American Dietetic Association, vol. 106, no. 3, pp. 403–413, 2006. View at Publisher · View at Google Scholar · View at Scopus
  113. E. Schievano, C. Finotello, E. De Angelis, S. Mammi, and L. Navarini, “Rapid authentication of coffee blends and quantification of 16-O-methylcafestol in roasted coffee beans by nuclear magnetic resonance,” Journal of Agricultural and Food Chemistry, vol. 62, no. 51, pp. 12309–12314, 2014. View at Publisher · View at Google Scholar · View at Scopus
  114. L. L. Monti, C. A. Bustamante, S. Osorio et al., “Metabolic profiling of a range of peach fruit varieties reveals high metabolic diversity and commonalities and differences during ripening,” Food Chemistry, vol. 190, pp. 879–888, 2016. View at Publisher · View at Google Scholar
  115. H.-S. Son, G.-S. Hwang, H.-J. Ahn, W.-M. Park, C.-H. Lee, and Y.-S. Hong, “Characterization of wines from grape varieties through multivariate statistical analysis of 1H NMR spectroscopic data,” Food Research International, vol. 42, no. 10, pp. 1483–1491, 2009. View at Publisher · View at Google Scholar · View at Scopus
  116. V. Gallo, P. Mastrorilli, I. Cafagna et al., “Effects of agronomical practices on chemical composition of table grapes evaluated by NMR spectroscopy,” Journal of Food Composition and Analysis, vol. 35, no. 1, pp. 44–52, 2014. View at Publisher · View at Google Scholar · View at Scopus
  117. R. Díaz, O. J. Pozo, J. V. Sancho, and F. Hernández, “Metabolomic approaches for orange origin discrimination by ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry,” Food Chemistry, vol. 157, pp. 84–93, 2014. View at Publisher · View at Google Scholar · View at Scopus
  118. L. R. Cagliani, N. Culeddu, M. Chessa, and R. Consonni, “NMR investigations for a quality assessment of Italian PDO saffron (Crocus sativus L.),” Food Control, vol. 50, pp. 342–348, 2015. View at Publisher · View at Google Scholar · View at Scopus
  119. F. Longobardi, A. Ventrella, C. Napoli et al., “Classification of olive oils according to geographical origin by using 1H NMR fingerprinting combined with multivariate analysis,” Food Chemistry, vol. 130, no. 1, pp. 177–183, 2012. View at Publisher · View at Google Scholar · View at Scopus
  120. F. Longobardi, D. Sacco, G. Casiello, A. Ventrella, and A. Sacco, “Characterization of the geographical and varietal origin of wheat and bread by means of nuclear magnetic resonance (NMR), isotope ratio mass spectrometry (IRMS) methods and chemometrics: a review,” Agricultural Sciences, vol. 6, no. 1, Article ID 53433, 2015. View at Publisher · View at Google Scholar
  121. E. Cubero-Leon, R. Peñalver, and A. Maquet, “Review on metabolomics for food authentication,” Food Research International, vol. 60, pp. 95–107, 2014. View at Publisher · View at Google Scholar · View at Scopus
  122. A. Trimigno, F. C. Marincola, N. Dellarosa, G. Picone, and L. Laghi, “Definition of food quality by NMR-based foodomics,” Current Opinion in Food Science, vol. 4, pp. 99–104, 2015. View at Publisher · View at Google Scholar
  123. L. Laghi, G. Picone, and F. Capozzi, “Nuclear magnetic resonance for foodomics beyond food analysis,” TrAC Trends in Analytical Chemistry, vol. 59, pp. 93–102, 2014. View at Publisher · View at Google Scholar · View at Scopus
  124. K. Dettmer, P. A. Aronov, and B. D. Hammock, “Mass spectrometry-based metabolomics,” Mass Spectrometry Reviews, vol. 26, no. 1, pp. 51–78, 2007. View at Publisher · View at Google Scholar · View at Scopus
  125. D. E. Garcia, E. E. Baidoo, P. I. Benke et al., “Separation and mass spectrometry in microbial metabolomics,” Current Opinion in Microbiology, vol. 11, no. 3, pp. 233–239, 2008. View at Publisher · View at Google Scholar · View at Scopus
  126. X. Li and C. Legido-Quigley, “Advances in separation science applied to metabonomics,” Electrophoresis, vol. 29, no. 18, pp. 3724–3736, 2008. View at Publisher · View at Google Scholar · View at Scopus
  127. H. J. Issaq, Q. N. Van, T. J. Waybright, G. M. Muschik, and T. D. Veenstra, “Analytical and statistical approaches to metabolomics research,” Journal of Separation Science, vol. 32, no. 13, pp. 2183–2199, 2009. View at Publisher · View at Google Scholar · View at Scopus
  128. J. Zhan, X.-J. Yu, Y.-Y. Zhong et al., “Generic and rapid determination of veterinary drug residues and other contaminants in raw milk by ultra performance liquid chromatography-tandem mass spectrometry,” Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 906, pp. 48–57, 2012. View at Publisher · View at Google Scholar · View at Scopus
  129. A. Malachová, M. Sulyok, E. Beltrán, F. Berthiller, and R. Krska, “Optimization and validation of a quantitative liquid chromatography—tandem mass spectrometric method covering 295 bacterial and fungal metabolites including all regulated mycotoxins in four model food matrices,” Journal of Chromatography A, vol. 1362, pp. 145–156, 2014. View at Publisher · View at Google Scholar · View at Scopus
  130. P. Mazzei and A. Piccolo, “1H HRMAS-NMR metabolomic to assess quality and traceability of mozzarella cheese from Campania buffalo milk,” Food Chemistry, vol. 132, no. 3, pp. 1620–1627, 2012. View at Publisher · View at Google Scholar · View at Scopus
  131. C. Siciliano, E. Belsito, R. De Marco, M. L. Di Gioia, A. Leggio, and A. Liguori, “Quantitative determination of fatty acid chain composition in pork meat products by high resolution 1H NMR spectroscopy,” Food Chemistry, vol. 136, no. 2, pp. 546–554, 2013. View at Publisher · View at Google Scholar · View at Scopus
  132. T. H. Soininen, N. Jukarainen, S. O. K. Auriola, R. Julkunen-Tiitto, R. Karjalainen, and J. J. Vepsäläinen, “Quantitative metabolite profiling of edible onion species by NMR and HPLC-MS,” Food Chemistry, vol. 165, pp. 499–505, 2014. View at Publisher · View at Google Scholar · View at Scopus
  133. Y. Zhao, P. Chen, L. Lin, J. M. Harnly, L. Yu, and Z. Li, “Tentative identification, quantitation, and principal component analysis of green pu-erh, green, and white teas using UPLC/DAD/MS,” Food Chemistry, vol. 126, no. 3, pp. 1269–1277, 2011. View at Publisher · View at Google Scholar · View at Scopus
  134. J. L. Gonçalves, J. A. Figueira, F. P. Rodrigues et al., “A powerful methodological approach combining headspace solid phase microextraction, mass spectrometry and multivariate analysis for profiling the volatile metabolomic pattern of beer starting raw materials,” Food Chemistry, vol. 160, pp. 266–280, 2014. View at Publisher · View at Google Scholar · View at Scopus
  135. F. Wei, K. Furihata, M. Koda, F. Hu, T. Miyakawa, and M. Tanokura, “Roasting process of coffee beans as studied by nuclear magnetic resonance: time course of changes in composition,” Journal of Agricultural and Food Chemistry, vol. 60, no. 4, pp. 1005–1012, 2012. View at Publisher · View at Google Scholar · View at Scopus
  136. C. Airoldi, E. Sironi, C. Dias et al., “Natural compounds against Alzheimer's disease: molecular recognition of Aβ1–42 peptide by Salvia sclareoides extract and its major component, rosmarinic acid, as investigated by NMR,” Chemistry, vol. 8, no. 3, pp. 596–602, 2013. View at Publisher · View at Google Scholar · View at Scopus
  137. E. Sironi, L. Colombo, A. Lompo et al., “Natural compounds against neurodegenerative diseases: molecular characterization of the interaction of catechins from green tea with Aβ1-42, PrP106-126, and ataxin-3 oligomers,” Chemistry—A European Journal, vol. 20, no. 42, pp. 13793–13800, 2014. View at Publisher · View at Google Scholar · View at Scopus
  138. G. Jiang, S. Lin, L. Wen et al., “Identification of a novel phenolic compound in litchi (Litchi chinensis Sonn.) pericarp and bioactivity evaluation,” Food Chemistry, vol. 136, no. 2, pp. 563–568, 2013. View at Publisher · View at Google Scholar · View at Scopus
  139. E. K. Kemsley, G. Le Gall, J. R. Dainty et al., “Multivariate techniques and their application in nutrition: a metabolomics case study,” British Journal of Nutrition, vol. 98, no. 1, pp. 1–14, 2007. View at Publisher · View at Google Scholar · View at Scopus
  140. T. Skov, A. H. Honoré, H. M. Jensen, T. Næs, and S. B. Engelsen, “Chemometrics in foodomics: handling data structures from multiple analytical platforms,” Trends in Analytical Chemistry, vol. 60, pp. 71–79, 2014. View at Publisher · View at Google Scholar
  141. M. Labra, G. Moriondo, A. Schneider et al., “Biodiversity of grapevines (Vitis vinifera L.) grown in the Aosta valley,” Vitis, vol. 41, no. 2, pp. 89–92, 2002. View at Google Scholar · View at Scopus
  142. F. De Mattia, S. Imazio, F. Grassi et al., “Genetic characterization of sardinia grapevine cultivars by SSR markers analysis,” Journal International des Sciences de la Vigne et du Vin, vol. 41, no. 4, pp. 175–184, 2007. View at Google Scholar · View at Scopus
  143. R. Godelmann, F. Fang, E. Humpfer et al., “Targeted and nontargeted wine analysis by 1H NMR spectroscopy combined with multivariate statistical analysis. Differentiation of important parameters: grape variety, geographical origin, year of vintage,” Journal of Agricultural and Food Chemistry, vol. 61, no. 23, pp. 5610–5619, 2013. View at Publisher · View at Google Scholar · View at Scopus
  144. N. A. Bokulich, C. M. L. Joseph, G. Allen, A. K. Benson, and D. A. Mills, “Next-generation sequencing reveals significant bacterial diversity of botrytized wine,” PLoS ONE, vol. 7, no. 5, Article ID e36357, 2012. View at Publisher · View at Google Scholar · View at Scopus
  145. N. A. Bokulich, M. Ohta, P. M. Richardson, and D. A. Mills, “Monitoring seasonal changes in winery-resident microbiota,” PLoS ONE, vol. 8, no. 6, Article ID e66437, 2013. View at Publisher · View at Google Scholar · View at Scopus
  146. V. David, S. Terrat, K. Herzine et al., “High-throughput sequencing of amplicons for monitoring yeast biodiversity in must and during alcoholic fermentation,” Journal of Industrial Microbiology & Biotechnology, vol. 41, no. 5, pp. 811–821, 2014. View at Publisher · View at Google Scholar · View at Scopus
  147. C. Agrimonti, M. Vietina, S. Pafundo, and N. Marmiroli, “The use of food genomics to ensure the traceability of olive oil,” Trends in Food Science & Technology, vol. 22, no. 5, pp. 237–244, 2011. View at Publisher · View at Google Scholar · View at Scopus
  148. I. Ganopoulos, C. Bazakos, P. Madesis, P. Kalaitzis, and A. Tsaftaris, “Barcode DNA high-resolution melting (Bar-HRM) analysis as a novel close-tubed and accurate tool for olive oil forensic use,” Journal of the Science of Food and Agriculture, vol. 93, no. 9, pp. 2281–2286, 2013. View at Publisher · View at Google Scholar · View at Scopus
  149. A. Yilmaz, N. T. Nyberg, P. Mølgaard, J. Asili, and J. W. Jaroszewski, “1H NMR metabolic fingerprinting of saffron extracts,” Metabolomics, vol. 6, no. 4, pp. 511–517, 2010. View at Publisher · View at Google Scholar · View at Scopus
  150. A. B. Uryupin and A. S. Peregudov, “Application of NMR techniques to the determination of the composition of tobacco, coffee, and tea products,” Journal of Analytical Chemistry, vol. 68, no. 12, pp. 1021–1032, 2013. View at Publisher · View at Google Scholar · View at Scopus
  151. J. Giacometti, A. B. Tomljanović, and D. Josić, “Application of proteomics and metabolomics for investigation of food toxins,” Food Research International, vol. 54, no. 1, pp. 1042–1051, 2013. View at Publisher · View at Google Scholar · View at Scopus
  152. T. J. Montville and M. L. Chikindas, “Biological control of foodborne bacteria,” in Food Microbiology: Fundamentals and Frontiers, M. P. Doyle and R. L. Buchanan, Eds., pp. 803–822, ASM Press, Washington, DC, USA, 4th edition, 2013. View at Google Scholar
  153. R. Caraballo, H. Dong, J. P. Ribeiro, J. Jiménez-Barbero, and O. Ramström, “Direct STD NMR identification of β-galactosidase inhibitors from a virtual dynamic hemithioacetal system,” Angewandte Chemie—International Edition, vol. 49, no. 3, pp. 589–593, 2010. View at Publisher · View at Google Scholar · View at Scopus
  154. C. Airoldi, S. Sommaruga, S. Merlo et al., “Targeting bacterial membranes: NMR spectroscopy characterization of substrate recognition and binding requirements of D-arabinose-5-phosphate isomerase,” Chemistry, vol. 16, no. 6, pp. 1897–1902, 2010. View at Publisher · View at Google Scholar · View at Scopus
  155. C. Airoldi, S. Giovannardi, B. La Ferla, J. Jiménez-Barbero, and F. Nicotra, “Saturation transfer difference NMR experiments of membrane Proteins in living cells under HR-MAS conditions: the interaction of the SGLT1 co-transporter with its ligands,” Chemistry—A European Journal, vol. 17, no. 48, pp. 13395–13399, 2011. View at Publisher · View at Google Scholar · View at Scopus
  156. C. Airoldi, A. Palmioli, A. D'Urzo et al., “Glucose-derived Ras pathway inhibitors: evidence of Ras-ligand binding and Ras-GEF (Cdc25) interaction inhibition,” ChemBioChem, vol. 8, no. 12, pp. 1376–1379, 2007. View at Publisher · View at Google Scholar · View at Scopus
  157. A. Palmioli, E. Sacco, C. Airoldi et al., “Selective cytotoxicity of a bicyclic Ras inhibitor in cancer cells expressing K-RasG13D,” Biochemical and Biophysical Research Communications, vol. 386, no. 4, pp. 593–597, 2009. View at Publisher · View at Google Scholar · View at Scopus
  158. A. Bordoni and F. Capozzi, “Foodomics for healthy nutrition,” Current Opinion in Clinical Nutrition and Metabolic Care, vol. 17, no. 5, pp. 418–424, 2014. View at Publisher · View at Google Scholar · View at Scopus
  159. S. Chadio and B. Kotsampasi, “The role of early life nutrition in programming of reproductive function,” Journal of Developmental Origins of Health and Disease, vol. 5, no. 1, pp. 2–15, 2014. View at Publisher · View at Google Scholar · View at Scopus
  160. S. M. Solon-Biet, S. J. Mitchell, R. de Cabo, D. Raubenheimer, D. G. Le Couteur, and S. Simpson, “Macronutrients and caloric intake in health and longevity,” Journal of Endocrinology, 2015. View at Publisher · View at Google Scholar
  161. L. Haddad, E. Achadi, M. A. Bendech et al., “The global nutrition report 2014: actions and accountability to accelerate the world's progress on nutrition,” The Journal of Nutrition, vol. 145, no. 4, pp. 663–671, 2015. View at Publisher · View at Google Scholar
  162. A. Cifuentes, “Food analysis and foodomics,” Journal of Chromatography A, vol. 1216, no. 43, p. 7109, 2009. View at Publisher · View at Google Scholar · View at Scopus
  163. M. Herrero, V. García-Cañas, C. Simo, and A. Cifuentes, “Recent advances in the application of capillary electromigration methods for food analysis and Foodomics,” Electrophoresis, vol. 31, no. 1, pp. 205–228, 2010. View at Publisher · View at Google Scholar · View at Scopus
  164. C. M. Williams, J. M. Ordovas, D. Lairon et al., “The challenges for molecular nutrition research 1: linking genotype to healthy nutrition,” Genes and Nutrition, vol. 3, no. 2, pp. 41–49, 2008. View at Publisher · View at Google Scholar · View at Scopus
  165. J. Wittwer, I. Rubio-Aliaga, B. Hoeft, I. Bendik, P. Weber, and H. Daniel, “Nutrigenomics in human intervention studies: current status, lessons learned and future perspectives,” Molecular Nutrition & Food Research, vol. 55, no. 3, pp. 341–358, 2011. View at Publisher · View at Google Scholar · View at Scopus
  166. C. E. Smith, J. M. Ordovás, C. Sánchez-Moreno, Y.-C. Lee, and M. Garaulet, “Apolipoprotein A-II polymorphism: relationships to behavioural and hormonal mediators of obesity,” International Journal of Obesity, vol. 36, no. 1, pp. 130–136, 2012. View at Publisher · View at Google Scholar · View at Scopus
  167. D. Corella, D. K. Arnett, K. L. Tucker et al., “A high intake of saturated fatty acids strengthens the association between the fat mass and obesity-associated gene and BMI,” The Journal of Nutrition, vol. 141, no. 12, pp. 2219–2225, 2011. View at Publisher · View at Google Scholar · View at Scopus
  168. A. Valdés, C. Simó, C. Ibáñez et al., “Effect of dietary polyphenols on K562 leukemia cells: a foodomics approach,” Electrophoresis, vol. 33, no. 15, pp. 2314–2327, 2012. View at Publisher · View at Google Scholar · View at Scopus
  169. C. Ibáñez, A. Valdés, V. García-Cañas et al., “Global Foodomics strategy to investigate the health benefits of dietary constituents,” Journal of Chromatography A, vol. 1248, pp. 139–153, 2012. View at Publisher · View at Google Scholar · View at Scopus
  170. U. Anđelković, T. Martinović, and D. Josić, “Foodomic investigations of food allergies,” Current Opinion in Food Science, vol. 4, pp. 92–98, 2015. View at Publisher · View at Google Scholar
  171. D. Rešetar, S. K. Pavelić, and D. Josić, “Foodomics for investigations of food toxins,” Current Opinion in Food Science, vol. 4, pp. 86–91, 2015. View at Publisher · View at Google Scholar
  172. K. S. Vimaleswaran, C. I. Le Roy, and S. P. Claus, “Foodomics for personalized nutrition: how far are we?” Current Opinion in Food Science, vol. 4, pp. 129–135, 2015. View at Publisher · View at Google Scholar
  173. G. H. Beaton, J. Burema, and C. Ritenbaugh, “Errors in the interpretation of dietary assessments,” The American Journal of Clinical Nutrition, vol. 65, no. 4, pp. 1100S–1107S, 1997. View at Google Scholar · View at Scopus
  174. S. L. Casperson, J. Sieling, J. Moon, L. Johnson, J. N. Roemmich, and L. Whigham, “A mobile phone food record app to digitally capture dietary intake for adolescents in a free-living environment: usability study,” JMIR mHealth and uHealth, vol. 3, no. 1, article e30, 2015. View at Publisher · View at Google Scholar
  175. M. J. Hutchesson, M. E. Rollo, R. Callister, and C. E. Collins, “Self-monitoring of dietary intake by young women: online food records completed on computer or smartphone are as accurate as paper-based food records but more acceptable,” Journal of the Academy of Nutrition and Dietetics, vol. 115, no. 1, pp. 87–94, 2015. View at Publisher · View at Google Scholar · View at Scopus
  176. W. Zhang, Q. Yu, B. Siddiquie, A. Divakaran, and H. Sawhney, “‘Snap-n-Eat’ food recognition and nutrition estimation on a smartphone,” Journal of Diabetes Science and Technology, vol. 9, no. 3, pp. 525–533, 2015. View at Publisher · View at Google Scholar
  177. C. Behnke and S. Seo, “Using smartphone technology to assess the food safety practices of farmers’ market foodservice employees,” Journal of Foodservice Business Research, vol. 18, no. 1, pp. 1–19, 2015. View at Publisher · View at Google Scholar