BioMed Research International / 2015 / Article / Fig 3

Research Article

Third-Degree Hindpaw Burn Injury Induced Apoptosis of Lumbar Spinal Cord Ventral Horn Motor Neurons and Sciatic Nerve and Muscle Atrophy in Rats

Figure 3

Double immunofluorescence staining of TUNEL and the Schwann cell marker S100 in the ipsilateral sciatic nerve in different groups. The number of TUNEL+ cells in the sciatic nerve increased significantly after the burn injury at 4 and 8 weeks compared with that in the sham burn group. Immunolabeling of S100 revealed that the number of Schwann cells in the sciatic nerve decreased significantly at 4 and 8 weeks after the burn injury compared with that in the sham burn group. The arrowheads indicate the TUNEL/S100 double-positive cells. After the burn injury, the percentage of merge cells in TUNEL+/S100+ increased significantly in the sciatic nerve. Data are plotted as mean ± SEM. , versus the sham burn group.