Third-Degree Hindpaw Burn Injury Induced Apoptosis of Lumbar Spinal Cord Ventral Horn Motor Neurons and Sciatic Nerve and Muscle Atrophy in Rats
Double immunofluorescence staining of TUNEL and the Schwann cell marker S100 in the ipsilateral sciatic nerve in different groups. The number of TUNEL+ cells in the sciatic nerve increased significantly after the burn injury at 4 and 8 weeks compared with that in the sham burn group. Immunolabeling of S100 revealed that the number of Schwann cells in the sciatic nerve decreased significantly at 4 and 8 weeks after the burn injury compared with that in the sham burn group. The arrowheads indicate the TUNEL/S100 double-positive cells. After the burn injury, the percentage of merge cells in TUNEL+/S100+ increased significantly in the sciatic nerve. Data are plotted as mean ± SEM. , versus the sham burn group.