miR-193b Modulates Resistance to Doxorubicin in Human Breast Cancer Cells by Downregulating MCL-1

Figure 4

miR-193b sensitized MCF-7/DOXR cells to doxorubicin depending on the downregulation of MCL-1. (a) MCF-7/DOXR cells were cotransfected with miR-193b or NCO plus empty pcDNA3.1 or pcDNA3.1-MCL-1. MCL-1 mRNA and protein expression levels were assayed by qPCR and western blot, respectively, . (b) MCL-1 expression vector rescued the MCF-7/DOXR cells treated with doxorubicin (1.2 μg/mL) plus miR-193b (50 nM), suggesting miR-193b modulates doxorubicin resistance through targeting MCL-1. (c) Enforced expression of MCL-1 decreased the apoptosis induced by doxorubicin plus miR-193b. Apoptosis was measured using Annexin V/PI staining by flow cytometry. Data are expressed as the means ± SE (). .

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