Binding and Endocytosis of Bovine Hololactoferrin by the Parasite Entamoeba histolytica

Figure 1

E. histolytica growth in B-holo-Lf as an iron source. ((a), (b)) Growth kinetics through 96 h in bovine holo-Lf (B-holo-Lf). Amoebic cultures were synchronized (see Methods section), 10⁴ cells were inoculated into each medium, and viability was estimated every 24 h by trypan blue exclusion. ((c), (d)) Amoebas were grown for 48 h, through three passages, in the media indicated. Viability was measured by trypan blue exclusion. Data are means of three independent experiments performed in triplicate ± SD.

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