Binding and Endocytosis of Bovine Hololactoferrin by the Parasite Entamoeba histolytica

Figure 2

B-holo-Lf internalization in both variants of E. histolytica depends on time and energy. (a) Flow cytometry: amoebae were synchronized and incubated (2 × 10⁵ cells) at 37°C or 6°C with FITC-B-holo-Lf for the periods of time indicated. The samples were then fixed with PFA and processed for fluorescence quantification. Data are means of three independent experiments performed in triplicate ± SD. (b) Confocal microscopy: amoebae were synchronized and 2 × 10⁵ cells were incubated at 37°C or 6°C with FITC-B-holo-Lf for the periods of time indicated. 1–3 and 7–9 indicate the nonvirulent amoebae and 4–6 and 10–12 indicate the virulent amoebae. Bar, 10 μm.

(a)

(b)