TOP2A Amplification and Overexpression in Hepatocellular Carcinoma Tissues

Figure 2

The disconcordance between TOP2A gene (TOP2A, red signal; CEP17, green signal) copy number detected by FISH and TOP2A protein (brown signal) expression detected by IHC in cases of HCC. (a) Case 34: FISH result showed TOP2A deletion (ratio = 0.65, original magnification ×1000) and negative TOP2A expression as shown in (b). (c) Case 32: FISH result showed TOP2A deletion (ratio = 0.64, original magnification ×1000) and TOP2A overexpression (30%) as shown in (d). (e) Case 25: FISH result showed normal TOP2A (ratio = 1.07, original magnification ×1000) and TOP2A overexpression (90%) as shown in (f).

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