In Vitro Gender-Dependent Inhibition of Porcine Cytochrome P450 Activity by Selected Flavonoids and Phenolic Acids

Figure 3

In vitro inhibition of CYP3A by myricetin and isorhamnetin in hepatic microsomes from male and female pigs. Data are presented as the mean percentage of remaining activity and standard error of the enzyme activity for three pools with microsomes from 2 male pigs in each pool and two pools with microsomes from 2 female pigs in each pool. (a) Effect of myricetin with (grey bars) and without (white bars) 5 min preincubation. ((b) and (c)) Saturation curve for 7-benzyloxy-4-trifluoromethylcoumarin O-dealkylation in hepatic microsomes from male (b) or female (c) pigs in the presence of myricetin. (d) Effect of isorhamnetin with (grey bars) and without (white bars) 5 min preincubation. ((e) and (f)) Saturation curve for 7-benzyloxy-4-trifluoromethylcoumarin O-dealkylation in hepatic microsomes from male (e) or female (f) pigs in the presence of isorhamnetin.

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