A Locked Nucleic Acid Probe Based on Selective Salt-Induced Effect Detects Single Nucleotide Polymorphisms

Figure 1

(a) The figure is showing the HRM curve for LNA probes with complementary template (complementary-curve) and the HRM curve for LNA probes with single-base mismatch template (the SM-curve). Complementary-curve (I) and SM-curve (II): in buffer with [Na⁺] 0.125 M; complementary-curve (III) and SM-curve (IV): in buffer with [Na⁺] 0.25 M; complementary-curve (V) and SM-curve (VI): in buffer with [Na⁺] 0.5 M; complementary-curve (VII) and SM-curve (VIII): in buffer with [Na⁺] 1 M. (b) The figure is showing the complementary curves and SM curves for probes without the LNA modification, and naming methods for these curves were the same as that of (a).

(a)

(b)