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BioMed Research International
Volume 2015, Article ID 391070, 6 pages
http://dx.doi.org/10.1155/2015/391070
Research Article

A Locked Nucleic Acid Probe Based on Selective Salt-Induced Effect Detects Single Nucleotide Polymorphisms

Department of Pharmacology, School of Pharmacy, China Medical University, Liaoning Key Laboratory of Molecular Targeted Anti-Tumor Drug Development and Evaluation, No. 77 Puhe Road, Shenbei District, Shenyang 110013, China

Received 23 May 2015; Accepted 27 July 2015

Academic Editor: Min Zhou

Copyright © 2015 Jing Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

(1) “NCBI-BLAST results show that the theoretical length of PCR product is 105 bp and the sequence is the only match to target gene. Therefore, the primer design is correct, and the location of the mutations in the sequence of PCR products.”

(2) “Agarose gel electrophoresis showed that only one DNA band is located near the 100 bp DNA mark. The results indicated we have the expected DNA products by PCR.”

(3) “These 4 figures are all of 4 cases in the gene sequencing results, they represent the 3 kinds of gene mutations. Sequencing results showed no type A gene could be detected.

  1. Supplementary Material