Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2015, Article ID 392872, 22 pages
http://dx.doi.org/10.1155/2015/392872
Review Article

Current and New Approaches in GMO Detection: Challenges and Solutions

1Platform of Biotechnology and Molecular Biology (PBB) and Biosafety and Biotechnology Unit (SBB), Scientific Institute of Public Health (WIV-ISP), J. Wytsmanstraat 14, 1050 Brussels, Belgium
2Technology and Food Sciences Unit, Institute for Agricultural and Fisheries Research (ILVO), Burg. Van Gansberghelaan 115, Bus 1, 9820 Merelbeke, Belgium
3Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium
4Department of Plant Biotechnology and Bioinformatics, Faculty of Sciences, Ghent University, Technologiepark 927, 9052 Ghent, Belgium

Received 17 July 2015; Accepted 7 September 2015

Academic Editor: Yiannis Kourkoutas

Copyright © 2015 Marie-Alice Fraiture et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Citations to this Article [49 citations]

The following is the list of published articles that have cited the current article.

  • Waqar Hassan, Nadia Jamil, Shehzaib Siddiqui, Anam Ali, Owais Quadri, Maliha Wajeeh, Aftab Ahmed Khan, and Saifullah Khan. View at Publisher · View at Google Scholar
  • Theo W. Prins, Richard A. van Hoof, Ingrid M. J. Scholtens, and Esther J. Kok, “Novel TaqMan PCR screening methods for element cry3A and construct gat/T-pinII to support detection of both known and unknown GMOs,” European Food Research and Technology, 2016. View at Publisher · View at Google Scholar
  • Jeroen P. van Dijk, Jianxin Shi, Alfred J. Arulandhu, David Dobnik, Arne Holst-Jensen, Jana Zel, and Esther J. Kok, “DNA enrichment approaches to identify unauthorized genetically modified organisms (GMOs),” Analytical and Bioanalytical Chemistry, vol. 408, no. 17, pp. 4575–4593, 2016. View at Publisher · View at Google Scholar
  • Bingfu Guo, Yong Guo, Huilong Hong, and Li-Juan Qiu, “Identification of Genomic Insertion and Flanking Sequence of G2-EPSPS and GAT Transgenes in Soybean Using Whole Genome Sequencing Method,” Frontiers in Plant Science, vol. 7, 2016. View at Publisher · View at Google Scholar
  • Jia Ge, Lijun Wang, Chen Yang, Lingyu Ran, Mengling Wen, Xianan Fu, Di Fan, and Keming Luo, “Intein-mediated Cre protein assembly for transgene excision in hybrid progeny of transgenic Arabidopsis,” Plant Cell Reports, 2016. View at Publisher · View at Google Scholar
  • Marko Bohanec, Biljana Mileva Boshkoska, Theo W. Prins, and Esther J. Kok, “SIGMO: A decision support System for Identification of genetically modified food or feed products,” Food Control, 2016. View at Publisher · View at Google Scholar
  • Marie-Alice Fraiture, Nancy H.C. Roosens, Isabel Taverniers, Marc De Loose, Dieter Deforce, and Philippe Herman, “Biotech Rice: Current developments and future detection challenges in food and feed chain,” Trends in Food Science & Technology, 2016. View at Publisher · View at Google Scholar
  • Linfu Zhou, Rui Wu, Xiaodan Shi, Dongyun Feng, Guodong Feng, Yining Yang, Wen Dai, Ting Bian, Tingting Liu, Ying He, Ming Shi, and Gang Zhao, “Simultaneous Detection of Five Pathogens from Cerebrospinal Fluid Specimens Using Luminex Technology,” International Journal of Environmental Research and Public Health, vol. 13, no. 2, pp. 193, 2016. View at Publisher · View at Google Scholar
  • Luca Braglia, Silvia Gianì, Diego Breviario, Floriana Gavazzi, Francesco Mastromauro, and Laura Morello, “Development and validation of the modular Feed-code method for qualitative and quantitative determination of feed botanical composition,” Analytical and Bioanalytical Chemistry, 2016. View at Publisher · View at Google Scholar
  • M.-A. Fraiture, S. Broeders, P. Herman, I. Taverniers, M. De Loose, D. Deforce, and N.H. Roosens, “Advances in the Identification of Genetically Modified Foods,” Advances in Food Authenticity Testing, pp. 543–563, 2016. View at Publisher · View at Google Scholar
  • Jinrong Wan, Li Song, Yalei Wu, Pius Brzoska, David Keys, Caifu Chen, Babu Valliyodan, J. Grover Shannon, and Henry T. Nguyen, “Application of Digital PCR in the Analysis of Transgenic Soybean Plants,” Advances in Bioscience and Biotechnology, vol. 07, no. 10, pp. 403–417, 2016. View at Publisher · View at Google Scholar
  • Dong-Sheng Lian, and Hua-Song Zeng, “Capillary Electrophoresis Based on Nucleic Acid Detection as Used in Food Analysis,” Comprehensive Reviews in Food Science and Food Safety, 2017. View at Publisher · View at Google Scholar
  • Anna Nadal, Marzia De Giacomo, Ralf Einspanier, Gijs Kleter, Esther Kok, Sarah McFarland, Roberta Onori, Alain Paris, Mònica Toldrà, Jeroen van Dijk, Jean-Michel Wal, and Maria Pla, “Exposure of livestock to GM feeds: Detectability and measurement,” Food and Chemical Toxicology, 2017. View at Publisher · View at Google Scholar
  • A. L. Van Eenennaam, and A. E. Young, “Detection of dietary DNA, protein, and glyphosate in meat, milk, and eggs1,” Journal of Animal Science, vol. 95, no. 7, pp. 3247–3269, 2017. View at Publisher · View at Google Scholar
  • Ingrid M. J. Scholtens, Bonnie Molenaar, Richard A. van Hoof, Stephanie Zaaijer, Theo W. Prins, and Esther J. Kok, “Semiautomated TaqMan PCR screening of GMO labelled samples for (unauthorised) GMOs,” Analytical and Bioanalytical Chemistry, 2017. View at Publisher · View at Google Scholar
  • Rong Li, Sheng Quan, Xiaofang Yan, Sukumar Biswas, Dabing Zhang, and Jianxin Shi, “Molecular characterization of genetically-modified crops: Challenges and strategies,” Biotechnology Advances, vol. 35, no. 2, pp. 302–309, 2017. View at Publisher · View at Google Scholar
  • C.L. Manzanares-Palenzuela, J.P. Martín-Clemente, M.J. Lobo-Castañón, and B. López-Ruiz, “Electrochemical detection of magnetically-entrapped DNA sequences from complex samples by multiplexed enzymatic labelling: Application to a transgenic food/feed quantitative survey,” Talanta, vol. 164, pp. 261–267, 2017. View at Publisher · View at Google Scholar
  • Marie-Alice Fraiture, Philippe Herman, Marc De Loose, Frédéric Debode, and Nancy H. Roosens, “How Can We Better Detect Unauthorized GMOs in Food and Feed Chains?,” Trends in Biotechnology, 2017. View at Publisher · View at Google Scholar
  • Ravil R. Garafutdinov, Aizilya A. Galimova, and Assol R. Sakhabutdinova, “Polymerase chain reaction with nearby primers,” Analytical Biochemistry, vol. 518, pp. 126–133, 2017. View at Publisher · View at Google Scholar
  • Sarah Z. Agapito-Tenfen, and Fern Wickson, “Challenges for transgene detection in landraces and wild relatives: learning from 15 years of debate over GM maize in Mexico,” Biodiversity and Conservation, 2017. View at Publisher · View at Google Scholar
  • Marie-Alice Fraiture, Philippe Herman, Nina Papazova, Marc De Loose, Dieter Deforce, Tom Ruttink, and Nancy H. Roosens, “An integrated strategy combining DNA walking and NGS to detect GMOs,” Food Chemistry, vol. 232, pp. 351–358, 2017. View at Publisher · View at Google Scholar
  • Zhenyu Yun, Liping Peng, Shengnan Huo, Xin Wu, Jun Zhou, Hongguang Zhang, Wenjing Li, Cai Qi, and Guangping Huang, “Auto-microfluidic thin-film chip for genetically modified maize detection,” Food Control, vol. 80, pp. 360–365, 2017. View at Publisher · View at Google Scholar
  • Ibrahim B. Salisu, Ahmad A. Shahid, Amina Yaqoob, Qurban Ali, Kamran S. Bajwa, Abdul Q. Rao, and Tayyab Husnain, “Molecular Approaches for High Throughput Detection and Quantification of Genetically Modified Crops: A Review,” Frontiers in Plant Science, vol. 8, 2017. View at Publisher · View at Google Scholar
  • R. Reeves, and Martin Phillipson, “Mass Releases of Genetically Modified Insects in Area-Wide Pest Control Programs and Their Impact on Organic Farmers,” Sustainability, vol. 9, no. 1, pp. 59, 2017. View at Publisher · View at Google Scholar
  • Van Eenennaam, and Young, “Detection of dietary DNA, protein, and glyphosate in meat, milk, and eggs,” Journal of Animal Science, vol. 95, no. 7, pp. 3247–3269, 2017. View at Publisher · View at Google Scholar
  • Sheng-ce Tao, Jiaying Hu, Rong Li, Shujuan Guo, Peng Liu, Litao Yang, Ning Shao, Jianwei Chen, Dabing Zhang, and Junhou Hui, “Visual detection of multiple genetically modified organisms in a capillary array,” Lab on a Chip, vol. 17, no. 3, pp. 521–529, 2017. View at Publisher · View at Google Scholar
  • D. Josić, Ž. Peršurić, D. Rešetar, T. Martinović, L. Saftić, and S. Kraljević Pavelić, “Use of Foodomics for Control of Food Processing and Assessing of Food Safety,” 2017. View at Publisher · View at Google Scholar
  • Geoffrey Cottenet, Carine Blancpain, Véronique Sonnard, and Poh Fong Chuah, “Two FAST multiplex real-time PCR reactions to assess the presence of genetically modified organisms in food,” Food Chemistry, 2018. View at Publisher · View at Google Scholar
  • Melissa Shizue de A. Yamashita, and Eduardo O. Melo, “Mucin 2 (MUC2) promoter characterization: an overview,” Cell and Tissue Research, 2018. View at Publisher · View at Google Scholar
  • John Emerson Leguizamón Guerrero, Andrés Felipe Vela Rojas, María Mercedes Arias Cortés, and Luis Felipe Cifuentes Fernández, “Panorama general de los organismos genéticamente modificados en Colombia y en el mundo: Capacidad nacional de detección,” Revista Colombiana de Biotecnología, vol. 20, no. 2, pp. 101–116, 2018. View at Publisher · View at Google Scholar
  • Martha G. Rocha-Munive, Mario Soberón, Saúl Castañeda, Esteban Niaves, Enrique Scheinvar, Luis E. Eguiarte, David Mota-Sánchez, Enrique Rosales-Robles, Urbano Nava-Camberos, José L. Martínez-Carrillo, Carlos A. Blanco, Alejandra Bravo, and Valeria Souza, “Evaluation of the Impact of Genetically Modified Cotton After 20 Years of Cultivation in Mexico,” Frontiers in Bioengineering and Biotechnology, vol. 6, 2018. View at Publisher · View at Google Scholar
  • Marie-Alice Fraiture, Julie Vandamme, Philippe Herman, and Nancy H. C. Roosens, “Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes,” BMC Biotechnology, vol. 18, no. 1, 2018. View at Publisher · View at Google Scholar
  • Jacchia Sara, Kagkli Dafni-Maria, Lievens Antoon, Angers-Loustau Alexandre, Savini Christian, Emons Hendrik, and Mazzara Marco, “Identification of single target taxon-specific reference assays for the most commonly genetically transformed crops using digital droplet PCR,” Food Control, 2018. View at Publisher · View at Google Scholar
  • Tigst Demeke, and David Dobnik, “Critical assessment of digital PCR for the detection and quantification of genetically modified organisms,” Analytical and Bioanalytical Chemistry, 2018. View at Publisher · View at Google Scholar
  • Welison Andrade Pereira, Alisson Santos Lopes da Silva, Danúbia Aparecida Costa Nobre, Guilherme de Sousa Paula, and Felipe Lopes da Silva, “Performance of transgenic and conventional soybean plants subjected to bioassay for detection of glyphosate tolerant seeds,” Crop Breeding and Applied Biotechnology, vol. 18, no. 1, pp. 39–46, 2018. View at Publisher · View at Google Scholar
  • Marie-Alice Fraiture, Dieter Deforce, Nancy H.C. Roosens, Assia Saltykova, Stefan Hoffman, Raf Winand, Kevin Vanneste, and Sigrid C.J. De Keersmaecker, “Nanopore sequencing technology: A new route for the fast detection of unauthorized GMO,” Scientific Reports, vol. 8, no. 1, 2018. View at Publisher · View at Google Scholar
  • Cheng Qian, Rui Wang, Hui Wu, Jianfeng Ping, and Jian Wu, “Recent advances in emerging DNA-based methods for genetically modified organisms (GMOs) rapid detection,” TrAC Trends in Analytical Chemistry, 2018. View at Publisher · View at Google Scholar
  • Sapna Jain, Mukesh K. Yadav, and Ajay Kumar, “Techniques for Production and Quality Assessment of Genetically Modified Foods,” Genetically Engineered Foods, pp. 177–202, 2018. View at Publisher · View at Google Scholar
  • Florin Iordache, Horia Maniu, Carmen Curutiu, Petruta Cornea, and Aneta Pop, “Identification of Genetically Modified Foods,” Genetically Engineered Foods, pp. 369–383, 2018. View at Publisher · View at Google Scholar
  • Bihter Avsar, Sahl Sadeghi, Aydin Turkec, and Stuart J. Lucas, “Identification and quantitation of genetically modified (GM) ingredients in maize, rice, soybean and wheat-containing retail foods and feeds in Turkey,” Journal of Food Science and Technology, 2019. View at Publisher · View at Google Scholar
  • Yuan-Kai Tu, Yen-Chun Lin, Yu-Wei Feng, Yeu-Yang Tseng, and Han-Wei Chen, “Visual, sensitive and rapid event-specific detection of genetically modified potato EH92-527-1 by loop-mediated isothermal amplification method,” Bioscience, Biotechnology, and Biochemistry, pp. 1–10, 2019. View at Publisher · View at Google Scholar
  • Yulou Qiu, Pan Li, Beibei Liu, Yuan Liu, Yulong Wang, Tingting Tao, Junli Xu, Bruce D. Hammock, Xianjin Liu, Rongfa Guan, and Cunzheng Zhang, “Phage-displayed nanobody based double antibody sandwich chemiluminescent immunoassay for the detection of Cry2A toxin in cereals,” Food and Agricultural Immunology, vol. 30, no. 1, pp. 924–936, 2019. View at Publisher · View at Google Scholar
  • Kiran Siddique, Jiaojun Wei, Rong Li, Dabing Zhang, and Jianxin Shi, “Identification of T-DNA Insertion Site and Flanking Sequence of a Genetically Modified Maize Event IE09S034 Using Next-Generation Sequencing Technology,” Molecular Biotechnology, 2019. View at Publisher · View at Google Scholar
  • Esther J. Kok, Debora CM. Glandorf, Theo W. Prins, and Richard GF. Visser, “Food and environmental safety assessment of new plant varieties after the European Court decision: process-triggered or product-based?,” Trends in Food Science & Technology, 2019. View at Publisher · View at Google Scholar
  • Lutz Grohmann, Jens Keilwagen, Nina Duensing, Emilie Dagand, Frank Hartung, Ralf Wilhelm, Joachim Bendiek, and Thorben Sprink, “Detection and Identification of Genome Editing in Plants: Challenges and Opportunities,” Frontiers in Plant Science, vol. 10, 2019. View at Publisher · View at Google Scholar
  • Gesche Spielmann, Sonja Ziegler, Gerhard Haszprunar, Ulrich Busch, Ingrid Huber, and Melanie Pavlovic, “Using loop-mediated isothermal amplification for fast species delimitation in eels (genus Anguilla), with special reference to the European eel (Anguilla anguilla),” Food Control, 2019. View at Publisher · View at Google Scholar
  • Anne-Laure Boutigny, Audrey Barranger, Claire De Boisséson, Yannick Blanchard, and Mathieu Rolland, “Targeted Next Generation Sequencing to study insert stability in genetically modified plants,” Scientific Reports, vol. 9, no. 1, 2019. View at Publisher · View at Google Scholar
  • Aurélie Bak, and Joanne B. Emerson, “Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection,” BMC Biotechnology, vol. 19, no. 1, 2019. View at Publisher · View at Google Scholar
  • Aqsa Akhtar, Zainab Rizvi, Muhammad Irfan, Asma Maqbool, Aftab Bashir, and Kauser Abdulla Malik, “Biochemical and morphological risk assessment of transgenic wheat with enhanced iron and zinc bioaccessibility,” Journal of Cereal Science, pp. 102881, 2019. View at Publisher · View at Google Scholar